Archives of Microbiology

, Volume 195, Issue 6, pp 419–429

Physiological roles of mycothiol in detoxification and tolerance to multiple poisonous chemicals in Corynebacterium glutamicum

  • Ying-Bao Liu
  • Ming-Xiu Long
  • Ya-Jie Yin
  • Mei-Ru Si
  • Lei Zhang
  • Zhi-Qiang Lu
  • Yao Wang
  • Xi-Hui Shen
Original Paper

DOI: 10.1007/s00203-013-0889-3

Cite this article as:
Liu, YB., Long, MX., Yin, YJ. et al. Arch Microbiol (2013) 195: 419. doi:10.1007/s00203-013-0889-3

Abstract

Mycothiol (MSH) plays important roles in maintaining cytosolic redox homeostasis and in adapting to reactive oxygen species in the high-(G + C)-content Gram-positive Actinobacteria. However, its physiological roles are ill defined compared to glutathione, the functional analog of MSH in Gram-negative bacteria and most eukaryotes. In this research, we explored the impact of intracellular MSH on cellular physiology by using MSH-deficient mutants in the model organism Corynebacterium glutamicum. We found that intracellular MSH contributes significantly to resistance to alkylating agents, glyphosate, ethanol, antibiotics, heavy metals and aromatic compounds. In addition, intracellular MSH is beneficial for withstanding oxidative stress induced by various oxidants in C. glutamicum. This study greatly expanded our current knowledge on the physiological functions of mycothiol in C. glutamicum and could be applied to improve the robustness of this scientifically and commercially important species in the future.

Keywords

Corynebacterium glutamicum Mycothiol Oxidative stress Detoxification 

Copyright information

© Springer-Verlag Berlin Heidelberg 2013

Authors and Affiliations

  • Ying-Bao Liu
    • 1
  • Ming-Xiu Long
    • 2
  • Ya-Jie Yin
    • 3
  • Mei-Ru Si
    • 1
  • Lei Zhang
    • 1
  • Zhi-Qiang Lu
    • 4
  • Yao Wang
    • 1
  • Xi-Hui Shen
    • 1
  1. 1.State Key Laboratory of Crop Stress Biology for Arid Areas and College of Life SciencesNorthwest A&F UniversityYanglingPeople’s Republic of China
  2. 2.College of Animal Science and TechnologyNorthwest A&F UniversityYanglingPeople’s Republic of China
  3. 3.Environmental Microbiology and Ecology Research Center, Chongqing Institute of Green and Intelligent TechnologyChinese Academy of SciencesChongqingPeople’s Republic of China
  4. 4.College of Plant ProtectionNorthwest A&F UniversityYanglingPeople’s Republic of China

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