Virulence determinants and biofilm production among Trueperella pyogenes recovered from abscesses of captive forest musk deer
- 518 Downloads
Trueperella pyogenes (formerly Arcanobacterium) is commonly isolated from domesticated or wild ruminants as an opportunistic pathogen. To investigate the role of virulence determinants (VDs) and biofilm production in T. pyogenes isolates, a total of 36 T. pyogenes were collected from abscesses of forest musk deer in Miyaluo Farm (Sichuan Province, China). The prevalence of VDs and associations with clonal types, antibiotic resistance and biofilm production were analyzed by PCR and bioassay. Finally, T. pyogenes isolates were separated into three clonal types based on the DNA fingerprinting of BOX-PCR. Isolates with less VDs obtained from sick forest musk deer were mainly belonged to Type 1, and the isolates with robust VD repertoire obtained from dead forest musk deer were included in Type 3. Accordingly, resistant isolates exhibited significant lower virulence than susceptible ones. Majority of T. pyogenes isolates of this study were capable of producing a biofilm. However, no VDs presence and antibiotic resistance were statistically associated with biofilm production. In conclusion, the current study demonstrated that T. pyogenes was probably the primary pathogen of abscesses in the forest musk deer. Moreover, as an animal origin pathogen, the increasing resistance of T. pyogenes isolates could also associate with a decreased virulence.
KeywordsTrueperella pyogenes Clonal type Virulence determinants Resistance Biofilm
This work was supported by the National Basic Research Program of China (973 Project: 2012CB722207) and National Forestry Bureau of China (2130211). The authors would like to acknowledge Xuxin Li from Miyaluo Farm for collecting the samples. We thank Kiswendsida Ouedeaogo and the anonymous reviewers for editing the manuscript.
Conflict of interest
The authors declare that they have no conflict of interest.
- Buntjer JB, Otsen M (2000) Cross checker provides computer-assisted marker interpretation. J Agric Genomics 4. http://www.ncgr.org/research/jag/papers99/paper599/indexp599.html
- Chen GL, Xu L, Yue BS, Zou FD (2007) Molecular cloning, characterizing of Interferon2 γ(IFN2γ) from forest musk deer (Moschus berezovskii) and its expression and purification in Escherichia coli. Sichuan J Zool 26:22–25Google Scholar
- CLSI (2009) Performance standards for antimicrobial susceptibility testing, 19th informational supplement, M100–S19. Clinical and Laboratory Standards Institute, WayneGoogle Scholar
- Johnson JR, Kuskowski MA, Owens K, Gajewski A, Winokur PL (2003) Phylogenetic origin and virulence genotype in relation to resistance to fluoroquinolones and/or extended-spectrum cephalosporins and cephamycins among Escherichia coli isolates from animals and humans. J Infect Dis 188:759–768PubMedCrossRefGoogle Scholar
- Martin B, Humbert O, Camara M, Guenzi E, Walker J, Mitchell T, Andrew P, Prudhomme M, Alloing G, Hakenbeck R, Morrison DA, Boulnois GJ, Claverys JP (1992) A highly conserved repeated DNA element located in the chromosome of Streptococcus pneumoniae. Nucleic Acids Res 20(Suppl. 13):3479–3483PubMedCrossRefGoogle Scholar
- Moreno E, Prats G, Sabaté M, Pérez T, Johnson JR, Andreu A (2006) Quinolone, fluoroquinolone and trimethoprim/sulfamethoxazole resistance in relation to virulence determinants and phylogenetic background among uropathogenic Escherichia coli. J Antimicrob Chemother 57:204–211PubMedCrossRefGoogle Scholar
- Natterman H, Horsch F (1977) The Corynebacterium pyogenes infection in cattle. 1. Incidence of the pathogen. Arch Exp Vet 31:405–413Google Scholar
- Singh DV, Matte MH, Matte GR, Jiang S, Sabeena F, Shukla BN, Sanyal SC, Huq A, Colwell RR (2001) Molecular analysis of Vibrio cholerae O1, O139, non-O1, and non-O139 strains: clonal relationship between clinical and environmental isolates. Appl Environ Microbiol 67(Suppl. 2):910–921PubMedCrossRefGoogle Scholar
- Syrmis MW, O’Carrol MR, Sloots TP, Coulter C, Wainwright CE, Bell SC, Nissen MD (2004) Rapid genotyping of Pseudomonas aeruginosa isolates harboured by adult and paediatric patients with cystic fibrosis using repetitive-element-based PCR assays. J Med Microbiol 53:1089–1096PubMedCrossRefGoogle Scholar
- Yassin AF, Hupfer H, Siering C, Schumann P (2011) Comparative chemotaxonomic and phylogenetic studies on the genus Arcanobacterium Collins et al. 1982 emend. Lehnen et al. 2006: proposal for Trueperella gen. nov. and emended description of the genus Arcanobacterium. Int J Syst Evol Microbiol 61:1265–1274PubMedCrossRefGoogle Scholar