Archives of Microbiology

, Volume 194, Issue 12, pp 1001–1012 | Cite as

Study on diversity of endophytic bacterial communities in seeds of hybrid maize and their parental lines

  • Yang Liu
  • Shan Zuo
  • Liwen Xu
  • Yuanyuan Zou
  • Wei SongEmail author
Original Paper


The seeds of plants are carriers of a variety of beneficial bacteria and pathogens. Using the non-culture methods of building 16S rDNA libraries, we investigated the endophytic bacterial communities of seeds of four hybrid maize offspring and their respective parents. The results of this study show that the hybrid offspring Yuyu 23, Zhengdan958, Jingdan 28 and Jingyu 11 had 3, 33, 38 and 2 OTUs of bacteria, respectively. The parents Ye 478, Chang 7-2, Zheng 58, Jing 24 and Jing 89 had 12, 36, 6, 12 and 2 OTUs, respectively. In the hybrid Yuyu 23, the dominant bacterium Pantoea (73.38 %) was detected in its female parent Ye 478, and the second dominant bacterium of Sphingomonas (26.62 %) was detected in both its female (Ye 478) and male (Chang 7-2) parent. In the hybrid Zhengdan 958, the first dominant bacterium Stenotrophomonas (41.67 %) was detected in both the female (Zheng 58) and male (Chang 7-2) parent. The second dominant bacterium Acinetobacter (9.26 %) was also the second dominant bacterium of its male parent. In the hybrid Jingdan 28, the second dominant bacterium Pseudomonas (12.78 %) was also the second dominant bacterium of its female parent, and its third dominant bacterium Sphingomonas (9.90 %) was the second dominant bacterium of its male parent and detected in its female parent. In the hybrid Jingyu 11, the first dominant bacterium Leclercia (73.85 %) was the third dominant bacterium of its male parent, and the second dominant bacterium Enterobacter (26.15 %) was detected in its male parent. As far as we know, this was the first research reported in China on the diversity of the endophytic bacterial communities of the seeds of various maize hybrids with different genotypes.


Hybrid maize Seed endophytic bacteria Bacterial diversity Culture-independent method 



Cetyltrimethylammonium bromide



This work was supported by the National Natural Science Foundation of China (No. 30770069), the Science Foundation of Beijing (No. 5092004). National Science and Technology Support Plans of China (2012BAK17B11) and International Science and Technology Cooperation Projects of Beijing (Z111105054611011). We would like to thank Professor Jiuran Zhao and Fengge Wang at Beijing Academy of Agriculture and Forestry Sciences for their assistance with supplying the seed sample of maize. We would also like to thank Christine Verhille at the University of British Columbia for her assistance with English language and grammatical editing of the manuscript.


  1. Adams PD, Kloepper JW (2002) Effect of host genotype on indigenous bacterial endophytes of cotton (Gossypium hirsutum L.). Plant Soil 240(1):181–189CrossRefGoogle Scholar
  2. Bacilio-Jiméne M, Aguilar-Flores S, del Valle MV, Pérez A, Zepeda A, Zenteno E (2001) Endophytic bacteria in rice seeds inhibit early colonization of roots by Azospirillum brasilense. Soil Biol Biochem 33:167–172CrossRefGoogle Scholar
  3. Barea JM, Pozo MJ, Azcon R, Azcon-Aguilar C (2005) Microbial co-operation in the rhizosphere. J Exp Bot 56(417):1761–1778PubMedCrossRefGoogle Scholar
  4. Cankar K, Kraigher H, Ravnikar M, Rupnik M (2005) Bacterial endophytes from seeds of Norway spruce (Picea abies L. Karst). FEMS Microbiol Lett 244:341–345PubMedCrossRefGoogle Scholar
  5. Chen G, Fan HW, Mao ZW, Liu CG (2009) Breeding and popularization of new maize variety Jingdan 28. Bull Agric Sci Technol 6:125–127 (in Chinese)Google Scholar
  6. Chun J, Lee JH, Jung Y, Kim M, Kim S, Kim BK, Lim YW (2007) EzTaxon: a web-based tool for the identification of prokaryotes based on 16S ribosomal RNA gene sequences. Int J Syst Evol Micr 57:2259–2261CrossRefGoogle Scholar
  7. Cottyn B, Regalado E, Lanoot B, De Cleene M, Mew TW, Swings J (2001) Bacterial populations associated with rice seed in the tropical environment. Phytopathology 91:282–292PubMedCrossRefGoogle Scholar
  8. Feng YJ, Song W (2001) Endophytic bacteria. Chin J Nat 23(5):249–252 (in Chinese)Google Scholar
  9. Feng YJ, Shen DL, Dong XZ, Song W (2003) In vitro symplasmata formation in the rice diazotrophic endophyte Pantoea agglomerans YS19. Plant Soil 255:435–444CrossRefGoogle Scholar
  10. Guan KL (2009) Seed physiological ecology. Chinese Agricultural Press, Beijing, pp 1–7 (in Chinese)Google Scholar
  11. Hardoim PR, van Overbeek LS, van Elsas JD (2008) Properties of bacterial endophytes and their proposed role in plant growth. Trends Microbiol 16:463–471PubMedCrossRefGoogle Scholar
  12. Jefferey SB, Daniel PR, Estelle RC (1999) Microbial community structure and function in the spermosphere as affected by soil and seed type. Can J Microbiol 45:138–144CrossRefGoogle Scholar
  13. Johnston-Monje D, Raizada MN (2011) Conservation and diversity of seed associated endophytes in Zea across boundaries of evolution, ethnography and ecology. PLoS ONE 6(6):e20396. doi: 10.1371/journal.pone.0020396
  14. Kloepper JW, Beauchamp CJ (1992) A review of issues related to measuring colonization of plant roots by bacteria. Can J Microbiol 38:1219–1232CrossRefGoogle Scholar
  15. Liu Y, Wang H, Sun XL, Yang HL, Wang YS, Song W (2011) Study on mechanisms of colonization of nitrogen-fixing PGPB, Klebsiella pneumoniae NG14 on the root surface of rice and the formation of biofilm. Curr Microbiol 62:1113–1122PubMedCrossRefGoogle Scholar
  16. Lucy M, Reed E, Glick BR (2004) Applications of free living plant growth-promoting rhizobacteria. Antonie Van Leeuwenhoek 86:1–25PubMedCrossRefGoogle Scholar
  17. Michiels K, Vanderleyden J, Vangool A (1989) Azospirillum—Plant Root Associations—a review. Biol Fert Soils 8:356–368CrossRefGoogle Scholar
  18. Neal JL, Larson RI, Atkinson TG (1973) Changes in rhizosphere populations of selected physiological groups of bacteria related to substitution of specific pairs of chromosomes in spring wheat. Plant Soil 39:209–212CrossRefGoogle Scholar
  19. Nelson EB (2004) Microbial dynamics and interactions in the spermosphere. Ann Rev Phytipathool 42:271–309CrossRefGoogle Scholar
  20. Picard C, Bosco M (2006) Heterozygosis drives maize hybrids to select elite 2, 4-diacethylphloroglucinol-producing Pseudomonas strains among resident soil populations. FEMS Microbiol Ecol 58(2):193–204PubMedCrossRefGoogle Scholar
  21. Rijavec T, Lapanje A, Dermastia M, Rupnik M (2007) Isolation of bacterial endophytes from germinated maize kernels. Can J Microbiol 53:802–808PubMedCrossRefGoogle Scholar
  22. San XC, Sun KZ, Liu JB (2007) Cultivation of hybrid maize, Zhengdan 958. Mod Agric Sci Technol 2:73 (in Chinese)Google Scholar
  23. Simon HM, Smith KP, Dodsworth JA, Guenthner B, Handelsman J, Goodman RM (2001) Influence of tomato genotype on growth of inoculated and indigenous bacteria in the spermosphere. Appl Environ Microbiol 67(2):514–520PubMedCrossRefGoogle Scholar
  24. Sturz AV (1995) The role of endophytic bacteria during seed piece decay and potato tuberization. Plant Soil 175:257–263CrossRefGoogle Scholar
  25. Sun HC, Wan JH, Niu YF (2005) Breeding and popularization of maize variety Yuyu 23 with high-yield, good-quality, and multi-resistant. J Maize Sci 13:95–96 (in Chinese)Google Scholar
  26. Sun L, Qiu FB, Zhang X, Dai X, Dong XZ, Song W (2008) Endophytic bacterial diversity in rice (Oryza sativa L.) roots estimated by 16S rDNA sequence analysis. Microb Ecol 55(3):415–424PubMedCrossRefGoogle Scholar
  27. van Overbeek L, van Elsas JD (2008) Effects of plant genotype and growth stage on the structure of bacterial communities associated with potato (Solanum tuberosum L.). FEMS Microbiol Ecol 64:283–296PubMedCrossRefGoogle Scholar
  28. Videira SS, de Araujo JL, da Silva Rodrigues L, Baldani VL, Baldani JI (2009) Occurrence and diversity of nitrogen-fixing Sphingomonas bacteria associated with rice plants grown in Brazil. FEMS Microbiol Lett 293(1):11–19PubMedCrossRefGoogle Scholar
  29. Wei G, Kloepper JW, Tuzun S (1996) Induced systemic resistance to cucumber diseases and increased plant growth by plant growth-promoting rhizobacteria under field conditions. Phytopathology 86:221–224CrossRefGoogle Scholar
  30. Xiao J, Li J, Yuan L, Tanksley SD (1995) Dominance is the major genetic basis of heterosis in rice as revealed by QTL analysis using molecular markers. Genetics 140(2):745–754PubMedGoogle Scholar
  31. Zhang L, Birch RG (1997) The gene for albicidin detoxification from Pantoea dispersa encodes an esterase and attenuates pathogenicity of Xanthomonas albilineans to sugarcane. Proc Natl Acad Sci 94:9984–9989PubMedCrossRefGoogle Scholar
  32. Zou YY, Liu Y, Wang JH, Song W (2011) Advances in plant seed-associated microbial ecology. Acta Ecol Sin 31(10):2906–2914 (in Chinese)Google Scholar

Copyright information

© Springer-Verlag 2012

Authors and Affiliations

  • Yang Liu
    • 1
    • 3
    • 4
  • Shan Zuo
    • 1
  • Liwen Xu
    • 2
  • Yuanyuan Zou
    • 1
  • Wei Song
    • 1
    Email author
  1. 1.College of Life SciencesCapital Normal UniversityBeijingPeople’s Republic of China
  2. 2.Maize Research CenterBeijing Academy of Agriculture and Forestry SciencesBeijingPeople’s Republic of China
  3. 3.China National Research Institute of Food and Fermentation IndustriesBeijingPeople’s Republic of China
  4. 4.China Center of Industrial Culture CollectionChina National Research Institute of Food and Fermentation IndustriesBeijingPeople’s Republic of China

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