Archives of Microbiology

, Volume 194, Issue 9, pp 785–794 | Cite as

Thiofractor thiocaminus gen. nov., sp. nov., a novel hydrogen-oxidizing, sulfur-reducing epsilonproteobacterium isolated from a deep-sea hydrothermal vent chimney in the Nikko Seamount field of the northern Mariana Arc

  • Hiroko MakitaEmail author
  • Satoshi Nakagawa
  • Masayuki Miyazaki
  • Ko-ichi Nakamura
  • Fumio Inagaki
  • Ken Takai
Original Paper


A novel chemolithoautotrophic hydrogen-oxidizing and sulfur-reducing bacterium, strain 496ChimT, was isolated from a deep-sea hydrothermal vent chimney collected from the hydrothermal field at the summit of Nikko Seamount field, in the Mariana Arc. Cells were rods or curved rods, motile by means of a single polar flagellum. Growth was observed between 15 and 45 °C (optimum 37 °C; doubling time, 2.1 h) and between pH 5.3 and 8.0 (optimum pH 6.0). The isolate was a strictly anaerobic, obligate chemolithoautotroph capable of growth using molecular hydrogen as the sole energy source, carbon dioxide as the sole carbon source, ammonium or nitrate as the sole nitrogen source, and elemental sulfur as the electron acceptor. The G+C content of genomic DNA was 35 mol%. Phylogenetic analysis based on 16S rRNA gene sequences indicated that the new isolate belonged to the class Epsilonproteobacteria, but the isolate was distantly related to the previously described Epsilonproteobacteria species potentially at the genus level (<90 %). On the basis of its physiological and molecular characteristics, strain 496ChimT (=DSM 22050Τ = JCM 15747Τ = NBRC 105224Τ) represents the sole species of a new genus, Thiofractor, for which the name Thiofractor thiocaminus is proposed.


Epsilonproteobacteria Hydrogen-oxidizing Sulfur-reducing Chemolithoautotroph Hydrothermal field Mariana 





Dimethyl sulfoxide


Trimethyl amine oxide



We would like to thank the captain and the crew of R/V Natsushima and ROV Hyper-Dolphin for helping to obtain deep-sea hydrothermal vent samples. We are grateful to Dr. Katsuyuki Uematsu for assistance with the preparation of electron micrographs and to Ms. Akane Tatedou and Mr. Nathan Johncock for help with nomenclature. This work was partially supported by the Institute for fermentation (IFO) and grant-in-aid from the Ministry Education, Culture, Sports, Science & Technology of Japan (No. 22760646).

Supplementary material

203_2012_814_MOESM1_ESM.docx (125 kb)
Supplementary Fig. S1 Time-course of production of hydrogen sulfide in the liquid phase (■) and concomitant bacterial growth (●) of strain 496ChimT. (DOCX 124 kb)
203_2012_814_MOESM2_ESM.docx (538 kb)
Supplementary Fig. S2 Polar lipid profiles of strain 496ChimT after two-dimensional TLC. DPG, diphosphatidylglycerol; PE, phosphatidylethanolamine; PS, phosphatidylserine; PL, phospholipids; and L1-2, unknown lipid. (DOCX 538 kb)


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Copyright information

© Springer-Verlag 2012

Authors and Affiliations

  • Hiroko Makita
    • 1
    Email author
  • Satoshi Nakagawa
    • 1
    • 2
  • Masayuki Miyazaki
    • 1
  • Ko-ichi Nakamura
    • 3
  • Fumio Inagaki
    • 4
  • Ken Takai
    • 1
  1. 1.Subsurface Geobiology and Advanced Research Project, Extremobiosphere Research Program, Institute of BiogeosciencesJapan Agency for Marine-Earth Science and Technology (JAMSTEC)YokosukaJapan
  2. 2.Laboratory of Microbiology, Faculty of Fisheries SciencesHokkaido UniversityHakodateJapan
  3. 3.National Institute of Advanced Industrial Science and TechnologyTsukubaJapan
  4. 4.Geomicrobiology Group, Kochi Institute for Core Sample ResearchJapan Agency for Marine-Earth Science and Technology (JAMSTEC)NankokuJapan

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