Archives of Microbiology

, Volume 194, Issue 9, pp 795–802 | Cite as

A TonB-dependent outer membrane receptor of Pseudomonas fluorescens: virulence and vaccine potential

Short Communication

Abstract

Pseudomonas fluorescens is a Gram-negative bacterium and a common aquaculture pathogen. In this study, we identified from a pathogenic P. fluorescens strain a TonB-dependent outer membrane receptor, TdrA, as a secreted protein and examined its function and vaccine potential. TdrA is composed of 746 residues and possesses conserved structural domains of TonB-dependent outer membrane receptors. Quantitative real-time reverse transcriptase-PCR analysis showed that expression of tdrA was upregulated under conditions of iron starvation and during infection of host cells. Consistently, iron depletion induced increased production of TdrA protein in the outer membrane. Compared to the wild type, a tdrA-knock out mutant (1) was unable to grow in the absence of iron, (2) exhibited drastically attenuated overall bacterial virulence, and (3) was impaired in the ability to establish lethal infection in host tissues. Purified recombinant TdrA (rTdrA), when used as a subunit vaccine to immunize flounder, was able to induce strong protective immunity, including production of serum-specific antibodies that resulted in effective protection against lethal-dose P. fluorescens challenge. Together, these results indicate that TdrA is an outer membrane receptor and a protective immunogen that is likely to be involved in iron acquisition and, as a result, required for optimal bacterial virulence.

Keywords

Pseudomonas fluorescens TonB-dependent receptor Virulence Vaccine 

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Copyright information

© Springer-Verlag 2012

Authors and Affiliations

  1. 1.Key Laboratory of Experimental Marine Biology, Institute of OceanologyChinese Academy of SciencesQingdaoPeople’s Republic of China
  2. 2.Hangzhou Key Laboratory for Animal Adaptation and Evolution, College of Life and Environmental SciencesHangzhou Normal UniversityHangzhouPeople’s Republic of China
  3. 3.Graduate University of the Chinese Academy of SciencesBeijingPeople’s Republic of China

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