Archives of Microbiology

, Volume 194, Issue 6, pp 541–548 | Cite as

Role of anionic charges of osmoregulated periplasmic glucans of Salmonella enterica serovar Typhimurium SL1344 in mice virulence

  • Arvind A. Bhagwat
  • Porteen Kannan
  • Yi Ning Leow
  • Mahesh Dharne
  • Allen Smith
Original Paper
First Online:
Received:
Revised:
Accepted:

Abstract

opgB gene of Salmonella enterica serovar Typhimurium was identified earlier in a genome-wide screen for mice virulence (Valentine et al. in Infect Immun 66:3378–3383, 1998). Although mutation in opgB resulted in avirulent Salmonella strain, how this gene contributes to pathogenesis remains unclear. Based on DNA homology, opgB is predicted to be responsible for adding phosphoglycerate residues to osmoregulated periplasmic glucans (OPGs) giving them anionic characteristics. In Escherichia coli, yet another gene, opgC, is also reported to contribute to anionic characteristics of OPGs by adding succinic acid residues. We constructed opgB, opgC, and opgBC double mutants of S. enterica serovar Typhimurium strain SL1344. As predicted opgBC mutant synthesized neutral OPGs that were devoid of any anionic substituents. However, opgB, opgC, and opgBC mutations had no significant impact on mice virulence as well as on competitive organ colonization. In low osmotic conditions, opgB, opgC, and opgBC mutants exhibited delay in growth initiation in the presence of sodium deoxycholate. Anionic substituents of OPGs from Salmonella although appear to be needed to overcome resistance of deoxycholate in hypoosmotic growth media, no evidence was found for their role in mice virulence.

Keywords

Periplasmicglucans Low osmolarity Bile salt sensitivity Salmonellosis Food microbiology Microbial food safety 
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Copyright information

© Springer-Verlag (Outside the USA) 2012

Authors and Affiliations

  • Arvind A. Bhagwat
    • 1
  • Porteen Kannan
    • 1
  • Yi Ning Leow
    • 1
    • 3
  • Mahesh Dharne
    • 1
  • Allen Smith
    • 2
  1. 1.Environmental Microbial and Food Safety Laboratory, Henry A. Wallace Beltsville Agricultural Research Center, Agricultural Research ServiceUSDABeltsvilleUSA
  2. 2.Diet Genomics and Immunology Laboratory, Henry A. Wallace Beltsville Agricultural Research Center, Agricultural Research ServiceUSDABeltsvilleUSA
  3. 3.Ngee Ann PolytechnicSingaporeSingapore

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