Archives of Microbiology

, Volume 194, Issue 7, pp 567–574 | Cite as

Phylogeny and photoheterotrophy in the acidophilic phototrophic purple bacterium Rhodoblastus acidophilus

Original Paper

Abstract

Norbert Pfennig isolated the first acidophilic purple bacterium over 40 years ago and named the organism Rhodopseudomonas acidophila (now Rhodoblastus acidophilus). Since the original work of Pfennig, no systematic study has been conducted on the phylogeny and carbon nutrition of a collection of strains of Rbl. acidophilus. We have isolated six new strains of Rbl. acidophilus from a Canadian peat bog. These strains, three of the original Pfennig strains and two additional putative R. acidophilus strains isolated several years ago in this laboratory, were characterized as to their pigments, phylogeny, and carbon sources supporting photoheterotrophic growth. Phototrophic cultures were either purple or orange in color, and the color of a particular strain was linked to phylogeny. As for the Pfennig strains of Rbl. acidophilus, all new strains grew photoheterotrophically at pH 5 on a variety of organic and fatty acids. However, in addition to methanol and ethanol, the new strains as well as the Pfennig strains grew on several other primary alcohols, results not reported in the original species description. Our work shows that some phylogenetic and physiological diversity exists within the species Rbl. acidophilus and supports the observation that few species of acidophilic purple bacteria appear to exist in nature.

Keywords

Purple nonsulfur bacteria Acidophiles Rhodoblastus acidophilus 

References

  1. Altschul SF, Madden TL, Schäffer AA, Zhang J, Zhang Z, Miller W, Lipman DJ (1997) Gapped BLAST and PSI-BLAST: a new generation of protein database search programs. Nucleic Acids Res 25:3389–3402PubMedCrossRefGoogle Scholar
  2. Bowman J (2005) Genus III Methylosinus. In: Brenner DJ, Krieg NR, Staley JT, Garrity GM (eds) Bergey’s manual of systematic bacteriology, vol 2, 2nd edn. Springer, New York, pp 417–420CrossRefGoogle Scholar
  3. Dedysh SN, Khmelenina VN, Suzina NE, Trotsenko YA, Semrau JD, Liesack W, Tiedje JM (2002) Methylocapsa acidiphila gen. nov., sp. nov., a novel methane-oxidizing and dinitrogen-fixing acidophilic bacterium from Sphagnum bog. Int J Syst Bacteriol 52:251–261Google Scholar
  4. Dedysh SN, Pankratov TA, Belova SE, Kulichevskaya IS, Liesack W (2006) Phylogenetic analysis and in situ identification of Bacteria community composition in an acidic Sphagnum peat bog. Appl Environ Microbiol 72:2110–2117PubMedCrossRefGoogle Scholar
  5. Douthit HA, Pfennig N (1976) Isolation and growth rates of methanol utilizing Rhodospirillaceae. Arch Microbiol 107:233–234CrossRefGoogle Scholar
  6. Felsenstein J (1989) PHYLIP—phylogeny inference package (version 3.2). Cladistics 5:164–166Google Scholar
  7. Gardiner AT, Cogdell RJ, Takaichi S (1993) The effect of growth conditions on the light harvesting apparatus in Rhodopseudomonas acidophila. Photo Res 38:159–167CrossRefGoogle Scholar
  8. Gorham E (1991) Northern peatlands: role in the carbon cycle and probable responses to climatic warming. Ecol Appl 1:182–195CrossRefGoogle Scholar
  9. Heinemeyer E-A, Schmidt K (1983) Changes in carotenoid biosynthesis caused by variations of growth conditions in cultures of Rhodopseudomonas acidophila strain 7050. Arch Microbiol 134:217–221CrossRefGoogle Scholar
  10. Imhoff JF (1995) Taxonomy and physiology of phototrophic purple bacteria and green sulfur bacteria. In: Blankenship RE, Madigan MT, Bauer CE (eds) Anoxygenic photosynthetic bacteria. Kluwer, Dordrecht, pp 1–15Google Scholar
  11. Imhoff JF (2001) Transfer of Rhodopseudomonas acidophila to the new genus Rhodoblastus as Rhodoblastus acidophilus gen. nov., comb. nov. Int J Syst Bacteriol 51:1863–1866Google Scholar
  12. Kennedy C (2005) Genus I. Beijerinckia. In: Brenner DJ, Krieg NR, Staley JT, Garrity GM (eds) Bergey’s manual of systematic bacteriology, vol 2, 2nd edn. Springer, New York, pp 423–432CrossRefGoogle Scholar
  13. Kulichevskaya IS, Guvez VS, Gorlenko VM, Liesack W, Dedysh SN (2006) Rhodoblastus sphagnicola sp. nov., a novel acidophilic purple non-sulfur bacterium from Sphagnum peat bog. Int J Syst Bacteriol 56:1397–1402Google Scholar
  14. Larkin MA, Blackshields G, Brown NP, Chenna R, McGettigan PA, McWilliam H, Valentin F, Wallace IM, Wilm A, Lopez R, Thompson JD, Gibson TJ, Higgins DG (2007) Clustal W and Clustal X version 2.0. Bioinformatics 23:2947–2948PubMedCrossRefGoogle Scholar
  15. Madigan MT (1988) Microbiology, physiology, and ecology of phototrophic bacteria. In: Zehnder AJB (ed) Biology of anaerobic microorganisms. John Wiley & Sons, New York, pp 39–111Google Scholar
  16. Madigan MT (2003) Anoxygenic phototrophic bacteria from extreme environments. Photosyn Res 76:157–171PubMedCrossRefGoogle Scholar
  17. Madigan MT, Gest H (1979) Growth of the photosynthetic bacterium Rhodopseudomonas capsulata chemoautotrophically in darkness with H2 as the energy source. J Bacteriol 137:524–530PubMedGoogle Scholar
  18. Madigan MT, Jung DO (2009) An overview of purple bacteria: systematics, physiology, and habitats. In: Hunter CN, Daldal F, Thurnauer MC, Beatty JT (eds) The purple phototrophic bacteria. Springer, Dordrecht, pp 1–15CrossRefGoogle Scholar
  19. Madigan MT, Imhoff JF, Trüper HG (2005) The Genus Rhodopila, Imhoff, Trüper and Pfennig 1984, 341VP. In: Garrity G (ed) Bergey’s manual of systematic bacteriology, vol 2, part C. Springer, New York, pp 83–85CrossRefGoogle Scholar
  20. Pfennig N (1969) Rhodopseudomonas acidophila, sp. n., a new species of the budding purple nonsulfur bacteria. J Bacteriol 99:597–602PubMedGoogle Scholar
  21. Pfennig N (1974) Rhodopseudomonas globiformis, sp. n. a new species of the Rhodospirillaceae. Arch Microbiol 100:197–206CrossRefGoogle Scholar
  22. Quayle JR, Pfennig N (1975) Utilization of methanol by Rhodospirillaceae. Arch Microbiol 102:193–198PubMedCrossRefGoogle Scholar
  23. Sahm H, Cox RB, Quayle JR (1976) Metabolism of methanol by Rhodopseudomonas acidophila. J Gen Microbiol 94:313–322PubMedGoogle Scholar
  24. Schmidt K (1971) Carotenoids of purple nonsulfur bacteria: composition and biosynthesis of the carotenoids of some strains of Rhodopseudomonas acidophila, Rhodospirillum tenue, and Rhodocyclus purpureus. Arch Microbiol 77:231–238CrossRefGoogle Scholar
  25. Schmidt K (1978) Biosynthesis of carotenoids. In: Clayton RK, Sistrom WR (eds) The photosynthetic bacteria. Plenum Press, New York, pp 729–750Google Scholar
  26. Schmidt K, Francis GW, Liaaen-Jensen S (1971) Bacterial carotenoids XXXVI. Remarkable C43-carotenoid artefacts of cross-conjugated carotenals and new carotenoid glucosides from Athiorhodaceae spp. Acta Chem Scan 25:2476–2486CrossRefGoogle Scholar
  27. Takaichi S (1999) Carotenoids and carotenogenesis in anoxygenic photosynthetic bacteria. In: Frank HA, Young AJ, Britton G, Cogdell R (eds) The photochemistry of carotenoids. Kluwer, Dordrecht, pp 39–69Google Scholar

Copyright information

© Springer-Verlag 2012

Authors and Affiliations

  1. 1.Department of MicrobiologySouthern Illinois UniversityCarbondaleUSA

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