Archives of Microbiology

, Volume 194, Issue 4, pp 269–279

Expression of multiple tfb genes in different Halobacterium salinarum strains and interaction of TFB with transcriptional activator GvpE

  • Anne Bleiholder
  • Regina Frommherz
  • Katharina Teufel
  • Felicitas Pfeifer
Original Paper

Abstract

Halobacterium salinarum NRC-1 contains multiple TBP and TFB proteins required for the recruitment of RNA polymerase for transcription initiation. The presence and the expression of genes encoding TFB were investigated in the two Hbt. salinarum strains NRC-1 and PHH1 and the mutant strain PHH4. The plasmid-encoded tfbC and tfbE genes of NRC-1 were lacking in PHH1 and PHH4. The 5′-end of the tfbF transcript was determined and contained a 5′-untranslated region of 39 nucleotides able to form a stem-loop structure. The expression of these tfb genes was studied in cultures growing at 15, 37°C and under heat shock conditions. Cold temperatures reduced growth and except for tfbF also the amounts of all tfb transcripts. However, the formation of gas vesicles increased in PHH1 and NRC-1. Heat shock reduced growth of PHH1 and NRC-1, but PHH4 was not affected. A 100-fold increase in tfbA and tfbB mRNA was observed in PHH1 and PHH4, whereas NRC-1 reduced the amounts of these transcripts and increased the expression of tfbG. All TFB proteins tested were able to interact with the transcription activator GvpE involved in gas vesicle formation that thus is able to recruit TFB to the gvp promoter.

Keywords

TFB proteins Genome variation Alternative tfb genes 

Abbreviations

TFB

Archaeal transcription factor

RNAP

RNA polymerase

qRT-PCR

Quantitative reverse transcriptase polymerase chain reaction

Supplementary material

203_2011_756_MOESM1_ESM.tif (2.9 mb)
Supplementary material 1 (TIFF 2931 kb)

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Copyright information

© Springer-Verlag 2011

Authors and Affiliations

  • Anne Bleiholder
    • 1
  • Regina Frommherz
    • 1
  • Katharina Teufel
    • 1
  • Felicitas Pfeifer
    • 1
  1. 1.Mikrobiologie und GenetikTechnische Universität DarmstadtDarmstadtGermany

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