Archives of Microbiology

, Volume 193, Issue 7, pp 497–513 | Cite as

Alleviation of cold stress in inoculated wheat (Triticum aestivum L.) seedlings with psychrotolerant Pseudomonads from NW Himalayas

  • Pankaj Kumar Mishra
  • Shekhar Chandra Bisht
  • Pooja Ruwari
  • Govindan Selvakumar
  • Gopal Krishna Joshi
  • Jaideep Kumar Bisht
  • Jagdish Chandra Bhatt
  • Hari Shankar Gupta
Original Paper


Twelve psychrotolerant Pseudomonad strains were selected on the basis of various plant growth-promoting (PGP) activities at cold temperature (4°C). The effect of inoculation with Pseudomonad strains on cold alleviation and growth of wheat seedling at cold temperature (8°C) was investigated under greenhouse condition. Inoculation with Pseudomonad strains significantly enhanced root/shoot biomass and nutrients uptake as compared to non-bacterized control at 60 days of plant growth. Bacterization significantly improved the level of cellular metabolites like chlorophyll, anthocyanin, free proline, total phenolics, starch content, physiologically available iron, proteins, and amino acids that are sign of alleviation of cold stress in wheat plants. Increased relative water content, reduced membrane injury (electrolyte leakage), and Na+/K+ ratio were also recorded in bacterized wheat plants. Electrolyte leakage and Na+/K+ were found inversely proportional to plant growth at cold temperature. Statistical analysis of twenty-three measured parameters revealed that uninoculated control was under cold stress while eight bacterial strains were positively alleviating cold stress in wheat plants. Thus, the psychrotrophic Pseudomonad strains could effectively provide a promising solution to overcome cold stress, which is major factor hindering wheat productivity under cold climatic condition.


Cold alleviation Wheat Psychrotrophic Pseudomonad PGPR NW Himalayas 



This research work was supported by Indian Council of Agricultural Research through the project “Application of Microorganisms in Agriculture and Allied Sectors (AMAAS)”, and our special thanks to Mr. Sanjay Kumar for his technical assistance during plant nutrient and soil analysis and Mr. K. K. S. Bisht, In-charge, Social Science Section, VPKAS, Almora, India, for the valuable suggestion during statistical analysis.


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Copyright information

© Springer-Verlag 2011

Authors and Affiliations

  • Pankaj Kumar Mishra
    • 1
  • Shekhar Chandra Bisht
    • 1
  • Pooja Ruwari
    • 1
  • Govindan Selvakumar
    • 2
  • Gopal Krishna Joshi
    • 3
  • Jaideep Kumar Bisht
    • 1
  • Jagdish Chandra Bhatt
    • 1
  • Hari Shankar Gupta
    • 4
  1. 1.Vivekananda Institute of Hill AgricultureIndian Council of Agricultural ResearchAlmoraIndia
  2. 2.Division of Soil SciencesIndian Institute of Horticultural ResearchBangaloreIndia
  3. 3.Department of BiotechnologyHNB Garhwal UniversitySrinagarIndia
  4. 4.Indian Agricultural Research Institute, (I.C.A.R.)New DelhiIndia

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