Archives of Microbiology

, Volume 192, Issue 11, pp 975–983 | Cite as

Organic acid production and plant growth promotion as a function of phosphate solubilization by Acinetobacter rhizosphaerae strain BIHB 723 isolated from the cold deserts of the trans-Himalayas

  • Arvind Gulati
  • Natasha Sharma
  • Pratibha Vyas
  • Swati Sood
  • Praveen Rahi
  • Vijaylata Pathania
  • Ramdeen Prasad
Short Communication
First Online:
Received:
Revised:
Accepted:

Abstract

An efficient phosphate-solubilizing plant growth–promoting Acinetobacter rhizosphaerae strain BIHB 723 exhibited significantly higher solubilization of tricalcium phosphate (TCP) than Udaipur rock phosphate (URP), Mussoorie rock phosphate (MRP) and North Carolina rock phosphate (NCRP). Qualitative and quantitative differences were discerned in the gluconic, oxalic, 2-keto gluconic, lactic, malic and formic acids during the solubilization of various inorganic phosphates by the strain. Gluconic acid was the main organic acid produced during phosphate solubilization. Formic acid production was restricted to TCP solubilization and oxalic acid production to the solubilization of MRP, URP and NCRP. A significant increase in plant height, shoot fresh weight, shoot dry weight, root length, root dry weight, and root, shoot and soil phosphorus (P) contents was recorded with the inoculated treatments over the uninoculated NP0K or NPTCPK treatments. Plant growth promotion as a function of phosphate solubilization suggested that the use of bacterial strain would be a beneficial addition to the agriculture practices in TCP-rich soils in reducing the application of phosphatic fertilizers.

Keywords

Acinetobacter rhizosphaerae Phosphate-solubilizing rhizobacteria Phosphate substrates Plant growth promotion Organic acids Maize 

Abbreviations

Ar

Acinetobacter rhizosphaerae

AMSL

Above mean sea level

NCBI

National Centre for Biotechnology Information

NCRP

North Carolina rock phosphate

PSRB

Phosphate-solubilizing rhizobacteria

SSP

Single super phosphate

TCP

Tricalcium phosphate

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Notes

Acknowledgments

Authors acknowledge the Director, Institute of Himalayan Bioresource Technology (CSIR), for providing the necessary facilities. Thanks are also due to Prof. (Dr.) Kamlesh Singh, Department of Statistics, Mathematics and Physics, CSK Himachal Pradesh Krishi Vishwavidyalaya (Agriculture University), Palampur and Dr. R. D. Singh, Biodiversity Division, Institute of Himalayan Bioresource Technology, Palampur, for advice on statistical analysis. The Council of Scientific and Industrial Research, Govt. of India, is also acknowledged for the financial support under the Council of Scientific and Industrial Research (CSIR) Network Project “Exploitation of India’s Rich Microbial Wealth” (NWP 006).

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Copyright information

© Springer-Verlag 2010

Authors and Affiliations

  • Arvind Gulati
    • 1
  • Natasha Sharma
    • 1
  • Pratibha Vyas
    • 1
  • Swati Sood
    • 1
  • Praveen Rahi
    • 1
  • Vijaylata Pathania
    • 2
  • Ramdeen Prasad
    • 3
  1. 1.Plant Pathology and Microbiology Laboratory, Hill Area Tea Science DivisionInstitute of Himalayan Bioresource Technology (CSIR)PalampurIndia
  2. 2.Analytical Laboratory, Natural Plant Product DivisionInstitute of Himalayan Bioresource Technology (CSIR)PalampurIndia
  3. 3.Soil Testing Laboratory, Hill Area Tea Science DivisionInstitute of Himalayan Bioresource Technology (CSIR)PalampurIndia

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