Archives of Microbiology

, Volume 192, Issue 6, pp 477–484 | Cite as

Saccharomyces cerevisiae strain UFMG 905 protects against bacterial translocation, preserves gut barrier integrity and stimulates the immune system in a murine intestinal obstruction model

  • Simone V. Generoso
  • Mirelle Viana
  • Rosana Santos
  • Flaviano S. Martins
  • José A. N. Machado
  • Rosa M. E. Arantes
  • Jacques R. Nicoli
  • Maria I. T. D. Correia
  • Valbert N. Cardoso
Original Paper

Abstract

Probiotic is a preparation containing microorganisms that confers beneficial effect to the host. This work assessed whether oral treatment with viable or heat-killed yeast Saccharomyces cerevisiae strain UFMG 905 prevents bacterial translocation (BT), intestinal barrier integrity, and stimulates the immunity, in a murine intestinal obstruction (IO) model. Four groups of mice were used: mice undergoing only laparotomy (CTL), undergoing intestinal obstruction (IO) and undergoing intestinal obstruction after previous treatment with viable or heat-killed yeast. BT, determined as uptake of 99mTc-E. coli in blood, mesenteric lymph nodes, liver, spleen and lungs, was significantly higher in IO group than in CTL group. Treatments with both yeasts reduced BT in blood and all organs investigated. The treatment with both yeasts also reduced intestinal permeability as determined by blood uptake of 99mTc-DTPA. Immunological data demonstrated that both treatments were able to significantly increase IL-10 levels, but only viable yeast had the same effect on sIgA levels. Intestinal lesions were more severe in IO group when compared to CTL and yeasts groups. Concluding, both viable and heat-killed cells of yeast prevent BT, probably by immunomodulation and by maintaining gut barrier integrity. Only the stimulation of IgA production seems to depend on the yeast viability.

Keywords

Probiotic Bacterial translocation Intestinal permeability Intestinal obstruction sIgA IL-10 

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Copyright information

© Springer-Verlag 2010

Authors and Affiliations

  • Simone V. Generoso
    • 1
  • Mirelle Viana
    • 1
  • Rosana Santos
    • 1
  • Flaviano S. Martins
    • 2
    • 3
  • José A. N. Machado
    • 5
  • Rosa M. E. Arantes
    • 4
  • Jacques R. Nicoli
    • 3
  • Maria I. T. D. Correia
    • 6
  • Valbert N. Cardoso
    • 1
  1. 1.Departamento De Análises Clínicas e Toxicológicas, Faculdade de FarmáciaUniversidade Federal de Minas GeraisBelo HorizonteBrazil
  2. 2.Departamento de Bioquímica e Imunologia, Instituto de Ciências BiológicasUniversidade Federal de Minas GeraisBelo HorizonteBrazil
  3. 3.Departamento de Microbiologia, Instituto de Ciências BiológicasUniversidade Federal de Minas GeraisBelo HorizonteBrazil
  4. 4.Departamento de Patologia Geral, Instituto de Ciências BiológicasUniversidade Federal de Minas GeraisBelo HorizonteBrazil
  5. 5.Hospital Santa CasaNúcleo de Pesquisa em Pós-GraduaçãoBelo HorizonteBrazil
  6. 6.Departamento de Cirurgia, Faculdade de MedicinaUniversidade Federal de Minas GeraisBelo HorizonteBrazil

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