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Archives of Microbiology

, Volume 192, Issue 6, pp 461–469 | Cite as

CalA, a cyAbrB protein, binds to the upstream region of ftsZ and is down-regulated in heterocysts in Anabaena sp. PCC 7120

  • Dongli He
  • Xudong Xu
Original Paper

Abstract

A protein of cyAbrB family of regulators, Alr0946 (CalA), was identified by DNA affinity chromatography using DNA fragments from the promoter region of ftsZ in Anabaena/Nostoc sp. PCC 7120. Electrophoretic mobility shift assays showed that the protein could bind to upstream regions of ftsZ (as biotin-labeled DNA fragments) and that the binding was inhibited by the same DNA fragments and a fragment upstream of hetC but not by 3 fragments upstream of glnA, hetR or ntcA. Both transcriptional fusion with gfp (green fluorescence protein) and Western blot analysis indicated that the expression of calA was down-regulated in heterocysts relative to vegetative cells. An insertional mutant of calA could not be completely segregated. Over-expression of calA in Anabaena/Nostoc caused no change in growth, heterocyst differentiation or expression of ftsZ. CalA as a DNA-binding protein is essential for the growth of Anabaena/Nostoc and may be required for the expression of ftsZ in vegetative cells.

Keywords

CalA ftsZ DNA-binding protein Anabaena 

Notes

Acknowledgments

This work was supported by the National Natural Science Foundation of China under grants 30570022 and 30770049.

Supplementary material

203_2010_573_MOESM1_ESM.pdf (89 kb)
Supplementary material 1 (PDF 89.1 kb)

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Copyright information

© Springer-Verlag 2010

Authors and Affiliations

  1. 1.The State Key Laboratory of Freshwater Ecology and Biotechnology, Institute of HydrobiologyChinese Academy of SciencesWuhan, HubeiChina

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