The putative Bacillus subtilis l,d-transpeptidase YciB is a lipoprotein that localizes to the cell poles in a divisome-dependent manner
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Cell wall synthesis in bacteria is spatially organized by cytoskeletal structures. Common to all cell wall-bearing bacteria, the cytokinetic machinery localizes the cell wall synthesis to the site of septation. Recently, MinJ, a new component of the cytokinetic machinery, or divisome, of Bacillus subtilis has been described. MinJ is part of the division site selection system but also essential for correct assembly of the divisome. Here, I used the isolated PDZ domain of MinJ for co-elution experiments. One of the proteins that co-eluted was the so far uncharacterized, putative l,d-transpeptidase protein YciB. Evidence is shown that YciB localizes to the cell poles. YciB localization depends on the existence of a mature divisome, suggesting that l,d-transpeptidases are, like penicillin-binding proteins, part of the divisome.
Keywordsl,d-transpeptidase YciB Cell division Protein localization Peptidoglycan synthesis Lipoprotein
Bovine serum albumin
Green fluorescent protein
Minimum inhibitory concentration
Polyacrylamide gel electrophoresis
Sodium dodecyl sulfate
I thank Anja Wittmann for the excellent technical support and Frank Bürmann for the kind gift of purified GFP-His. I thank Catriona Donovan for critical reading of the manuscript. Dr. Reinhard Krämer is acknowledged for his generous support, and funding by the Deutsche Forschungsgemeinschaft (DFG, SFB 635) is acknowledged.