Mixotrophic metabolism in Burkholderia kururiensis subsp. thiooxydans subsp. nov., a facultative chemolithoautotrophic thiosulfate oxidizing bacterium isolated from rhizosphere soil and proposal for classification of the type strain of Burkholderia kururiensis as Burkholderia kururiensis subsp. kururiensis subsp. nov.
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A thiosulfate-oxidizing facultative chemolithoautotrophic Burkholderia sp. strain ATSB13T was previously isolated from rhizosphere soil of tobacco plant. Strain ATSB13T was aerobic, Gram-staining-negative, rod shaped and motile by means of sub-terminal flagellum. Strain ATSB13T exhibited mixotrophic growth in a medium containing thiosulfate plus acetate. A phylogenetic study based on 16S rRNA gene sequence analysis indicated that strain ATSB13T was most closely related to Burkholderia kururiensis KP23T (98.7%), Burkholderia tuberum STM678T (96.5%) and Burkholderia phymatum STM815T (96.4%). Chemotaxonomic data [G+C 64.0 mol%, major fatty acids, C18:1 ω7c (28.22%), C16:1 ω7c/15 iso 2OH (15.15%), and C16:0 (14.91%) and Q-8 as predominant respiratory ubiquinone] supported the affiliation of the strain ATSB13T within the genus Burkholderia. Though the strain ATSB13T shared high 16S rRNA gene sequence similarity with the type strain of B. kururiensis but considerably distant from the latter in terms of several phenotypic and chemotaxonomic characteristics. DNA–DNA hybridization between strain ATSB13T and B. kururiensis KP23T was 100%, and hence, it is inferred that strain ATSB13T is a member of B. kururiensis. On the basis of data obtained from this study, we propose that B. kururiensis be subdivided into B. kururiensis subsp. kururiensis subsp. nov. (type strain KP23T = JCM 10599T = DSM 13646T) and B. kururiensis subsp. thiooxydans subsp. nov. (type strain ATSB13T = KACC 12758T).
KeywordsBurkholderia kururiensis subsp. kururiensis Burkholderia kururiensis subsp. thiooxydans Mixotrophic growth Reduced sulfur compounds oxidation Thiosulfate-oxidizing bacteria
RA thanks Rural Development Administration (RDA), Republic of Korea for awarding post doctoral fellowship. This study was supported by National Academy of Agricultural Science, RDA, Republic of Korea.
- Brenner DJ, Krieg NR, Staley JT (2005) The Proteobacteria. In: Garrity GM (ed) Bergey’s manual of systematic bacteriology. Springer, New York, pp 575–623Google Scholar
- Coenye T, Falsen E, Hoste B, Ohlén M, Goris J, Govan JRW, Gillis M, Vandamme P (2000) Description of Pandoraea gen. nov. with Pandoraea apista sp. nov., Pandoraea pulmonicola sp. nov., Pandoraea pnomenusa sp. nov., Pandoraea sputorum sp. nov., and Pandoraea norimbergensis comb. nov. Int J Syst Evol Microbiol 50:887–899PubMedGoogle Scholar
- Ezaki T, Hashimoto Y, Yabuuchi E (1989) Fluorometric deoxyribonucleic acid–deoxyribonucleic acid hybridization in micro-dilution wells as an alternative to membrane filter hybridization in which radioisotopes are used to determine genetic relatedness among bacterial strains. Int J Syst Bacteriol 39:224–229Google Scholar
- Gerhardt P, Murray RGE, Wood WA, Krieg NR (1994) Methods for general and molecular bacteriology. American Society for Microbiology, Washington, DCGoogle Scholar
- Jung SJ, Jang KH, Shin EH, Park SK, Park CH (2005) Characteristics of sulfur oxidation by a newly isolated Burkholderia spp. J Microbiol Biotechnol 15:716–721Google Scholar
- Katayama Y, Uchino Y, Wood AP, Kelly DP (2006) Confirmation of Thiomonas delicata (formerly Thiomonas delicatus) as a distinct species of the genus Thiomonas Moreira and Amils 1997 with comments on some species currently assigned to genus. Int J Syst Evol Microbiol 56:2553–2557CrossRefPubMedGoogle Scholar
- Kelly DP (1989) Physiology and biochemistry of unicellular sulfur bacteria. In: Schlegel HG, Bowien B (eds) Autotrophic bacteria. Springer, Berlin, pp 193–217Google Scholar
- Moore DD (1995) Preparation and analysis of DNA. In: Ausubel FW, Brent R, Kingston RE, Moore DD, Seidman JG, Smith JA, Struhl K (eds) Current protocols in molecular biology. Wiley, New York, pp 2–11Google Scholar
- Sasser M (1990) Identification of bacteria by gas chromatography of cellular fatty acids. MIDI Technical Note 101. Newark, DE: MIDIGoogle Scholar
- Viallard V, Poirier I, Cournoyer B, Haurat J, Wiebkin S, Ophel-Keller K, Balandreau J (1998) Burkholderia graminis sp. nov., a rhizospheric Burkholderia species, and reassessment of [Pseudomonas] phenazinium, [Pseudomonas] pyrrocinia and [Pseudomonas] glathei as Burkholderia. Int J Syst Evol Microbiol 48:549–563CrossRefGoogle Scholar
- Wittke R, Ludwig W, Peiffer S, Kleiner D (1997) Isolation and characterization of Burkholderia norimbergensis sp. nov., a mildly alkaliphilic sulfur oxidizer. Syst Appl Microbiol 20:549–553Google Scholar