Archives of Microbiology

, Volume 191, Issue 9, pp 721–733 | Cite as

Yeast protein phosphatases Ptp2p and Msg5p are involved in G1–S transition, CLN2 transcription, and vacuole morphogenesis

  • Hermansyah
  • Minetaka Sugiyama
  • Yoshinobu Kaneko
  • Satoshi Harashima
Original Paper
First Online:
Received:
Revised:
Accepted:

Abstract

We previously reported that double disruption of protein phosphatase (PPase) genes PTP2 (phosphotyrosine-specific PPase) and MSG5 (phosphotyrosine and phosphothreonine/serine-PPase) causes Ca2+ sensitive growth, whereas the single disruptions do not. This finding suggests that Ptp2p and Msg5p are involved in Ca2+-induced stress response in a redundant manner. To gain insight into the molecular mechanism causing calcium sensitivity of the ∆ptp2 ∆msg5 double disruptant, we performed fluorescence-activated cell sorting analysis and found a delayed G1 phase. This delayed G1 was consistent with the defect in bud emergence, and reduced CLN2 transcription upon addition of CaCl2. We also found that Slt2p is hyper-phosphorylated in the Δptp2 Δmsg5 double disruptant and that the vacuole of the Δptp2 Δmsg5 double disruptant is fragmented even in the absence of Ca2+. These findings suggest that both Ptp2p and Msg5p are involved in the G1 to S transition and vacuole morphogenesis possibly through their regulation of Slt2 pathway.

Keywords

Saccharomyces cerevisiae Protein phosphatase PTP2 MSG5 Calcium sensitivity Delayed G1 

Abbreviations

PPase

Protein phosphatase

MAPK

Mitogen-activated protein kinase

Cas

Calcium sensitive

CWI

Cell wall integrity

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Notes

Acknowledgment

This work was supported by a Grant-in-Aid for Scientific Research B, 2007 to 2009, to S.H. from the Ministry of Education, Science, Sports and Culture of Japan.

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Copyright information

© Springer-Verlag 2009

Authors and Affiliations

  • Hermansyah
    • 1
  • Minetaka Sugiyama
    • 1
  • Yoshinobu Kaneko
    • 1
  • Satoshi Harashima
    • 1
  1. 1.Department of Biotechnology, Graduate School of EngineeringOsaka UniversitySuita, OsakaJapan

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