Archives of Microbiology

, Volume 191, Issue 6, pp 493–500 | Cite as

Enzymatic, outer membrane proteins and plasmid alterations of starved Vibrio parahaemolyticus and Vibrio alginolyticus cells in seawater

  • Fethi Ben Abdallah
  • Héla Kallel
  • Amina Bakhrouf
Original Paper

Abstract

The marine bacteria Vibrio parahaemolyticus and V. alginolyticus were incubated in seawater for 8 months to evaluate their adaptative responses to starvation. The starved cells showed an altered biochemical and enzymatic profiles, respectively, on Api 20E and Api ZYM systems and an evolution to the filterable minicells state capable to pass membrane pore size 0.45 μm. Outer membrane proteins patterns of stressed bacteria were also altered. Indeed, these modifications were manifested by the appearance and/or disappearance of bands as well as in the level of expression of certain proteins. Plasmids profiles analysis showed that V. alginolyticus ATCC 33787 lost three plasmids, whereas other tested strains conserved their initial profiles.

Keywords

Vibrio Seawater Starvation Enzymatic Morphological Outer membrane proteins Plasmids Alterations 

Notes

Acknowledgments

We are grateful to M’SELMI Abdelhamid principal technical; USCR: Unité des Services Communs de Recherche, Faculté des Sciences de Tunis. Campus Universitaire. Tunisia.

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Copyright information

© Springer-Verlag 2009

Authors and Affiliations

  • Fethi Ben Abdallah
    • 1
    • 2
  • Héla Kallel
    • 2
  • Amina Bakhrouf
    • 1
  1. 1.Laboratoire d’Analyse, Traitement et Valorisation des Polluants de l’Environnement et des ProduitsFaculté de Pharmacie Rue AvicenneMonastirTunisia
  2. 2.Unité de Fermentation et de Développement de Vaccins VirologiquesInstitut Pasteur de TunisTunisTunisia

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