Abstract
Streptomyces globisporus 1912 produces a polyketide antibiotic landomycin E (LaE), which possesses anticancer activity. A 1.8 kb DNA fragment at the end of landomycin E biosynthetic gene cluster was sequenced. DNA sequence analysis of this fragment identified one complete open reading frame, designated lndW. The deduced sequence of lndW gene product revealed significant similarity to the ATP-binding domains of the ABC (ATP-binding protein cassette) superfamily of transport-related proteins. Knockout of lndW had no significant effect on resistance to LaE and its production. The expression of lndW in S. globisporus 1912 was proven via transcriptional fusion of lndW promoter to EGFP (enhanced green fluorescent protein). Overexpression of lndW in S. lividans TK24 conferred resistance to LaE. The mechanism of lndW function in LaE biosynthesis is discussed.
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Communicated by Jean-Luc Pernodet
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203_2008_367_MOESM1_ESM.ppt
Fig. S1. (a) Autofluorescence of 36h- and 48-h-old mycelia of S. globisporus 1912 (pIJ8660). (b) Temporal expression of PlndW::EGFP in 42h-, 48- and 66h-old mycelia of S. globisporus 1912(pIJ8660W). Transmission images are shown as black and white photographs. Fluorescence images are shown as color images. (PPT 2825 kb)
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Ostash, I., Rebets, Y., Ostash, B. et al. An ABC transporter encoding gene lndW confers resistance to landomycin E. Arch Microbiol 190, 105–109 (2008). https://doi.org/10.1007/s00203-008-0367-5
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DOI: https://doi.org/10.1007/s00203-008-0367-5