The molecular basis of salt adaptation in Methanosarcina mazei Gö1
The study on the molecular basis of salt adaptation and its regulation in archaea is still in its infancy, but genomics and functional genome analyses combined with classical biochemistry shed light on the processes conferring salt adaptation in the methanogenic archaeon Methanosarcina mazei Gö1. In this article, we will review discoveries made within the last years that will culminate in the description of the overall cellular response of M. mazei Gö1 to elevated salinities. This response includes accumulation of solutes and export of Na+ as well as potential uptake/export of K+ but also a restructuring of the cell surface.
KeywordsArchaea Methanogens Osmoregulation Salt adaptation Methanosarcina mazei Gö1
- Bakker EP (1992) Cell K+ and K+ transport systems in procaryotes. In: Bakker EP (ed) Alcali cation transport systems in procaryotes. CRC Press, Boca RatonGoogle Scholar
- Pflüger K, Baumann S, Gottschalk G, Lin W, Santos H, Müller V (2003) Lysine-2,3-aminomutase and β-lysine acetyltransferase genes of methanogenic archaea are salt induced and are essential for the biosynthesis of N ε-acetyl-β-lysine and growth at high salinity. Appl Environ Microbiol 69:6047–6055PubMedCrossRefGoogle Scholar
- Pflüger K, Wieland H, Müller V (2005) Osmoadaptation in methanogenic archaea: recent insights from a genomic perspective. In: Gunde-Cimerman N, Oren A, Plemenitas A (eds) Adaptation to life at hight salt concentrations in archaea, bacteria, and eukarya. Springer, Dordrecht, pp 241–251Google Scholar
- Saum SH, Sydow JF, Palm P, Pfeiffer F, Oesterhelt D, Müller V (2006) Biochemical and molecular characterization of the biosynthesis of glutamine and glutamate, two major compatible solutes in the moderately halophilic bacterium Halobacillus halophilus. J Bacteriol 188:6808–6815PubMedCrossRefGoogle Scholar
- Spanheimer R, Hoffmann M, Kögl S, Schmidt S, Pflüger K, Müller V (2008) Differential regulation of Ota and Otb, two primary glycine betaine transporters in the methanogenic archaeon Methanosarcina mazei Gö1. J Mol Microbiol Biotechnol (in press)Google Scholar