Archives of Microbiology

, Volume 190, Issue 1, pp 11–18

Different bacterial strategies to degrade taurocholate

  • Verena Rösch
  • Karin Denger
  • David Schleheck
  • Theo H. M. Smits
  • Alasdair M. Cook
Original Paper

DOI: 10.1007/s00203-008-0357-7

Cite this article as:
Rösch, V., Denger, K., Schleheck, D. et al. Arch Microbiol (2008) 190: 11. doi:10.1007/s00203-008-0357-7

Abstract

Aerobic enrichment cultures with taurocholate or alkanesulfonates as sole sources of carbon and energy for growth were successful and yielded nine bacterial isolates, all of which utilized taurocholate. Growth was complex and involved not only many, usually transient, excretion products but also sorption of taurocholate and cholate to cells. Three metabolic strategies to dissimilate taurocholate were elucidated, all of which involved bile salt hydrolase cleaving taurocholate to cholate and taurine. Comamonas testosteroni KF-1 utilized both the taurine and the cholate moieties for growth. Pseudomonas spp., e.g. strain TAC-K3 and Rhodococcus equi TAC-A1 grew with the cholate moiety and released taurine quantitatively. Delftia acidovorans SPH-1 utilized the taurine moiety and released cholate.

Keywords

Bile salt hydrolase Comamonas testosteroni Delftia acidovorans Desulfonation Excretion of cholate Excretion of taurine Pseudomonas spp. Taurine dissimilation 

Copyright information

© Springer-Verlag 2008

Authors and Affiliations

  • Verena Rösch
    • 1
  • Karin Denger
    • 1
  • David Schleheck
    • 1
    • 2
  • Theo H. M. Smits
    • 1
    • 3
  • Alasdair M. Cook
    • 1
  1. 1.Department of BiologyThe UniversityKonstanzGermany
  2. 2.School of Biotechnology and Biomolecular Sciences and Centre for Marine Biofouling and Bio-InnovationUniversity of New South WalesSydneyAustralia
  3. 3.Agroscope Changins-Wädenswil ACW, Swiss Federal Research StationWädenswilSwitzerland

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