Phage specificity and lipopolysaccarides of stem- and root-nodulating bacteria (Azorhizobium caulinodans, Sinorhizobium spp., and Rhizobiumspp.) of Sesbaniaspp.
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Phage susceptibility pattern and its correlation with lipopolysaccharide (LPS) and plasmid profiles may help in understanding the phenotypic and genotypic diversity among highly promiscuous group of rhizobia nodulating Sesbania spp.; 43 phages were from two stem-nodulating bacteria of S. rostrata and 16 phages were from root-nodulating bacteria of S. sesban, S. aegyptica and S. rostrata. Phage susceptibility pattern of 38 Sesbania nodulating bacteria was correlated with their LPS rather than plasmid profiles. Different species of bacteria (A. caulinodans- ORS571, SRS1-3 and Sinorhizobium saheli- SRR907, SRR912) showing distinct LPS subtypes were susceptible to different group of phages. Phages could also discriminate the strains of Si. saheli (SSR312, SAR610) possessing distinct LPS subtypes. Phages of Si. meliloti (SSR302) were strain-specific. All the strains of R. huautlense having incomplete LPS (insignificant O-chain) were phage-resistant. In in vitro assay, 100% of the phages were adsorbed to LPS of indicator bacterium or its closely related strain(s) only. These observations suggest the significance of LPS in phage specificity of Sesbania nodulating rhizobia. Highly specific phages may serve as biological marker for monitoring the susceptible bacterial strains in culture collections and environment.
KeywordsPhage specificity Lipopolysaccharide (LPS) Sesbania Sinorhizobium Rhizobium Azorhizobium caulinodans
- deLajudie P, Bogusz D (1984) Isolation and characterization of two bacteriophages of a stem-nodulating Rhizobium strain from Sesbania rostrata. Can J Microbiol 30:521–525Google Scholar
- Lindstrom K, Jarvis BDW, Lindström PE, Patel JJ (1983) DNA homology, phage-typing, and cross-nodulation studies of rhizobia infecting Galega species. Can J Microbiol 29:781–789Google Scholar
- Olsson JE, Rolfe BG (1985) Stem and root nodulation of the tropical legume Sesbania rostrata by Rhizobium strains ORS-571 and WE7. J Plant Physiol 121:199–210Google Scholar
- Olsson JE, Rolfe BG, Shine J, Plazinski J, Nayadu M, Ridge R, Dart P (1984) The biological characterization of Sesbania rostrata infection by Rhizobium species. In: Vegar C, Newton WE (eds) Advances in nitrogen fixation research. Nijhoff, Hague, p 715Google Scholar