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Archives of Microbiology

, Volume 188, Issue 5, pp 463–472 | Cite as

Genetic and proteomic analyses of CO utilization by Methanosarcina acetivorans

  • Michael Rother
  • Ellen Oelgeschläger
  • William W. MetcalfEmail author
Original Paper

Abstract

Methanosarcina acetivorans, a member of the methanogenic archaea, can grow with carbon monoxide (CO) as the sole energy source and generates, unlike other methanogens, substantial amounts of acetate and formate in addition to methane. Phenotypic analyses of mutant strains lacking the cooS1F operon and the cooS2 gene suggest that the monofunctional carbon monoxide dehydrogenase (CODH) system contributes to, but is not required for, carboxidotrophic growth of M. acetivorans. Further, qualitative proteomic analyses confirm a recent report (Lessner et al., Proc Natl Acad Sci USA, 103:17921–17926, 2006) in showing that the bifunctional CODH/acetyl-CoA synthase (ACS) system, two enzymes involved in CO2-reduction, and a peculiar protein homologous to both corrinoid proteins and methyltransferases are synthesized at elevated levels in response to CO; however, the finding that the latter protein is also abundant when trimethylamine serves as growth substrate questions its proposed involvement in the reduction of methyl-groups to methane. Potential catabolic mechanisms and metabolic adaptations employed by M. acetivorans to effectively utilize CO are discussed.

Keywords

Methanosarcina acetivorans Carbon monoxide Carbon monoxide dehydrogenase 

Notes

Acknowledgments

We are indebted to V. Müller, University of Frankfurt, for his support and for critical reading of the manuscript. This work was supported by grants from the Deutsche Forschungsgemeinschaft to M.R. (SPP1112) and from the Department of Energy (DE-FG02-02ER15296) to W. W. M.

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Copyright information

© Springer-Verlag 2007

Authors and Affiliations

  • Michael Rother
    • 1
  • Ellen Oelgeschläger
    • 1
  • William W. Metcalf
    • 2
    Email author
  1. 1.Institut für Molekulare Biowissenschaften, Abteilung Molekulare Mikrobiologie und BioenergetikJohann Wolfgang Goethe-Universität FrankfurtFrankfurt am MainGermany
  2. 2.Department of MicrobiologyUniversity of Illinois at Urbana-ChampaignUrbanaUSA

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