Archives of Microbiology

, Volume 188, Issue 4, pp 421–431

DNA bipyrimidine photoproduct repair and transcriptional response of UV-C irradiated Bacillus subtilis

  • Ralf Moeller
  • Erko Stackebrandt
  • Thierry Douki
  • Jean Cadet
  • Petra Rettberg
  • Hans-Joachim Mollenkopf
  • Günther Reitz
  • Gerda Horneck
Original Paper

Abstract

Vegetative wild-type and DNA repair-deficient (homologous recombination, recA and nucleotide excision repair, uvrB) Bacillus subtilis cells were exposed to UV-C radiation. Colony formation, DNA bipyrimidine photoproducts and gene expression were measured during cell recovery. Gene expression was measured after 60 min cell recovery where 50% (wild-type), 30% (recA) and 8% (uvrB), respectively, of the UV-C induced DNA photoproducts were repaired. We examined changes in the gene expression following UV exposure in wild-type and both repair-deficient strains. A set of known and unknown genes were found to be significantly up-regulated in wild-type B. subtilis cells, whereas no or lower gene induction was determined for both mutant strains. In addition, the possible roles of newly identified UV-responsive genes are discussed with respect to cellular recovery following exposure to UV irradiation.

Keywords

Bacillus subtilis UV-C radiation DNA repair Gene expression DNA photoproducts 

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Copyright information

© Springer-Verlag 2007

Authors and Affiliations

  • Ralf Moeller
    • 1
    • 2
  • Erko Stackebrandt
    • 2
  • Thierry Douki
    • 4
  • Jean Cadet
    • 4
  • Petra Rettberg
    • 1
  • Hans-Joachim Mollenkopf
    • 3
  • Günther Reitz
    • 1
  • Gerda Horneck
    • 1
  1. 1.German Aerospace Center (DLR), Radiation Biology DivisionInstitute of Aerospace MedicineCologneGermany
  2. 2.German Collection of Microorganisms and Cell Cultures GmbH (DSMZ)BraunschweigGermany
  3. 3.Max Planck Institute for Infection Biology (MPIIB), Microarray Core FacilityBerlinGermany
  4. 4.Département de Recherche Fondamentale sur la Matière Condensée CEA-GrenobleLaboratoire “Lésions des Acides Nucléiques”, Service de Chimie Inorganique et Biologique, UMR-E3 (CEA/UJF)Grenoble Cedex 9France

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