DNA bipyrimidine photoproduct repair and transcriptional response of UV-C irradiated Bacillus subtilis
- First Online:
Vegetative wild-type and DNA repair-deficient (homologous recombination, recA and nucleotide excision repair, uvrB) Bacillus subtilis cells were exposed to UV-C radiation. Colony formation, DNA bipyrimidine photoproducts and gene expression were measured during cell recovery. Gene expression was measured after 60 min cell recovery where 50% (wild-type), 30% (recA) and 8% (uvrB), respectively, of the UV-C induced DNA photoproducts were repaired. We examined changes in the gene expression following UV exposure in wild-type and both repair-deficient strains. A set of known and unknown genes were found to be significantly up-regulated in wild-type B. subtilis cells, whereas no or lower gene induction was determined for both mutant strains. In addition, the possible roles of newly identified UV-responsive genes are discussed with respect to cellular recovery following exposure to UV irradiation.
KeywordsBacillus subtilis UV-C radiation DNA repair Gene expression DNA photoproducts
- Au N, Kuester-Schoeck E, Mandava V, Bothwell LE, Canny SP, Chachu K, Colavito SA, Fuller SN, Groban ES, Hensley LA, O’Brien TC, Shah A, Tierney JT, Tomm LL, O’Gara TM, Goranov AI, Grossman AD, Lovett SM (2005) Genetic composition of the Bacillus subtilis SOS system. J Bacteriol 187:7655–7666PubMedCrossRefGoogle Scholar
- Friedberg EC, Walker GC, Siede W (1995) DNA repair and mutagenesis. ASM Press, Washington pp 698–735Google Scholar
- Sonenshein AL, Hoch JA, Losick R (2002) Appendix: functional classification of the Bacillus subtilis protein-encoding genes. In: Sonenshein AL, Hoch JA, Losick R (eds) Bacillus subtilis and its closest relatives: from genes to cells, Appendix 2. ASM Press, Washington, pp 570–615Google Scholar