Archives of Microbiology

, Volume 188, Issue 4, pp 411–419 | Cite as

Knockout of crtB or crtI gene blocks the carotenoid biosynthetic pathway in Deinococcus radiodurans R1 and influences its resistance to oxidative DNA-damaging agents due to change of free radicals scavenging ability

  • Lei Zhang
  • Qiao Yang
  • Xuesong Luo
  • Chengxiang Fang
  • Qiuju Zhang
  • Yali Tang
Original Paper

Abstract

Deinococcus radiodurans R1, a red-pigmented strain of the extremely radioresistant genus Deinococcus, contains a major carotenoid namely deinoxanthin. The high resistance of this organism against the lethal actions of DNA-damaging agents including ionizing radiation and ultraviolet light (UV) has been widely reported. However, the possible antioxidant role of carotenoids in this strain has not been completely elucidated. In this study, we constructed two colorless mutants by knockout of crtB and crtI genes, respectively. Comparative analysis of the two colorless mutants and the wild type showed that the two colorless mutants were more sensitive to ionizing radiation, UV, and hydrogen peroxide, but not to mitomycin-C (MMC). With electron spin resonance (ESR) and spin trapping techniques, we observed that hydroxyl radical signals occurred in the suspensions of UV irradiated Deinococcus radiodurans cells and the intensity of signals was influenced by carotenoids levels. We further showed that the carotenoid extract from the wild type could obviously scavenge superoxide anions generated by the irradiated riboflavin/EDTA system. These results suggest that carotenoids in D. radiodurans R1 function as free radical scavengers to protect this organism against the deleterious effects of oxidative DNA-damaging agents.

Keywords

Deinococcus radiodurans Carotenoids ESR Free radical 

Abbreviations

UV

Ultraviolet light

MMC

Mitomycin-C

ESR

Electron spin resonance

ROS

Reactive oxygen species

DMPO

5,5-Dimethyl-1-pyrroline-1-oxide

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Copyright information

© Springer-Verlag 2007

Authors and Affiliations

  • Lei Zhang
    • 1
  • Qiao Yang
    • 1
  • Xuesong Luo
    • 1
  • Chengxiang Fang
    • 1
  • Qiuju Zhang
    • 1
  • Yali Tang
    • 1
  1. 1.College of Life SciencesWuhan UniversityWuhanPeople’s Republic of China

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