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Archives of Microbiology

, 188:235 | Cite as

Cell surface structure enhancing uptake of polyvinyl alcohol (PVA) is induced by PVA in the PVA-utilizing Sphingopyxis sp. strain 113P3

  • Xiaoping Hu
  • Rie Mamoto
  • Yumi Shimomura
  • Kazuhide Kimbara
  • Fusako Kawai
Original Paper

Abstract

Polyvinyl alcohol (PVA)-utilizing Sphingopyxis sp. 113P3 (reidentified from Sphingomonas sp. 113P3) removed almost 0.5% PVA from culture supernatants in 4 days. Faster degradation of 0.5% PVA was performed by the periplasmic fraction. The average molecular size of PVA in the culture supernatant or cell-bound PVA was gradually shifted higher, suggesting that lower molecular size molecules are degraded faster. Depolymerized products were found in neither the culture supernatant nor the cell-bound fraction; however they were recovered from the periplasmic fraction. As extracellular or cell-associated PVA oxidase activity was almost undetectable in strain 113P3, degradation of PVA must be performed by periplasmic PVA dehydrogenase after uptake into the periplasm. Following the consumption of PVA, a dent appeared on the cell surface on day 2 and increased in size and depth for 4 days and was maintained for 8 days. Ultrastructural change on the cell surface was only observed in PVA medium, but not in nutrient broth (NB), suggesting that the change is induced by PVA. Fluorescein-4-isothiocyanate-labeled PVA was bound more to cells grown in PVA than to cells grown in NB. No binding was found with PVA-grown cells treated with formaldehyde. Thus, a dent on the cell surface seems to be related to the uptake of PVA.

Keywords

Polyvinyl alcohol Biodegradation of PVA Sphingopyxis sp. strain 113P3 Cell surface PVA-uptake structure 

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Copyright information

© Springer-Verlag 2007

Authors and Affiliations

  • Xiaoping Hu
    • 1
    • 2
  • Rie Mamoto
    • 3
  • Yumi Shimomura
    • 1
  • Kazuhide Kimbara
    • 1
  • Fusako Kawai
    • 1
  1. 1.Laboratory of Applied Microbiology, Research Institute for BioresourcesOkayama UniversityKurashikiJapan
  2. 2.College of Plant Protection and Shaanxi Key Laboratory of Molecular Biology for AgricultureNorthwest A&F UniversityYanglingPeople’s Republic of China
  3. 3.Faculty of NutritionKobe Gakuin UniversityKobeJapan

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