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Archives of Microbiology

, Volume 188, Issue 1, pp 15–25 | Cite as

Diversity of Microcystis aeruginosa isolates (Chroococcales, Cyanobacteria) from East-African water bodies

  • Sigrid HaandeEmail author
  • Andreas Ballot
  • Thomas Rohrlack
  • Jutta Fastner
  • Claudia Wiedner
  • Bente Edvardsen
Original Paper

Abstract

With exception of South Africa, very little is known about the presence and abundance of toxic cyanobacteria and cyanobacterial blooms on the African continent. The close proximity between society and nature, and the use of the sparse water resources as drinking water in large parts of Africa, lead to the recognition that more knowledge on toxic cyanobacterial blooms is of major importance. The bloom forming cyanobacterium Microcystis aeruginosa is known to produce cyclic heptatoxins (microcystins) which can be toxic to humans. In this study the morphological, genetic, and chemical characters of 24 strains of M. aeruginosa from several water bodies in Kenya and Uganda, some of them used as drinking water sources, were examined. The M. aeruginosa strains possessed different levels of diversity depending on characterisation method. Four morphotypes were identified based on the traditional morphological approach, 10 genotypes by DNA sequence comparison of the PC-IGS and ITS1 rDNA regions, and 10 chemotypes based on MALDI-TOF-MS oligopeptide analysis. Only 4 of the 24 isolated strains from East Africa were found to produce microcystins, while oligopeptides belonging to the aeruginosin and cyanopeptolin class were detected in most strains.

Keywords

Microcystis aeruginosa Microcystin Morphology ITS1-rDNA PC-IGS Phylogeny Matrix-assisted laser desorption/ionization time-of-flight MALDI-TOF mass spectrometry ELISA 

Notes

Acknowledgments

We are grateful to Randi Skulberg, Hege Hansen, and Monika Degebrodt for excellent laboratory assistance and Kjetil Røberg for helpful assistance with phylogenetic analysis. We are grateful to Marcel Erhard (AnagnosTec, Luckenwalde) and Martin Welker (Technical University, Berlin) for use of databases and for providing reference PSD spectra. We would like to thank the Kenyan Ministry of Education Science and Technology for providing research permission (No. MOEST 13/001/31 C90) and the Uganda National Council for Science and Technology for providing research permit (No. UNCST-EC584). This study was funded by the Norwegian Research Council (No. 148839/730). We thank the two anonymous reviewers for helpful comments.

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Copyright information

© Springer-Verlag 2007

Authors and Affiliations

  • Sigrid Haande
    • 1
    • 2
    Email author
  • Andreas Ballot
    • 3
  • Thomas Rohrlack
    • 1
  • Jutta Fastner
    • 4
  • Claudia Wiedner
    • 3
  • Bente Edvardsen
    • 1
    • 5
  1. 1.Norwegian Institute for Water ResearchOsloNorway
  2. 2.Department of BiologyUniversity of BergenBergenNorway
  3. 3.Department of Limnology of Stratified LakesLeibniz-Institute for Freshwater Ecology and Inland FisheriesStechlin-NeuglobsowGermany
  4. 4.Federal Environmental AgencyBerlinGermany
  5. 5.Department of BiologyUniversity of OsloOsloOsloNorway

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