Archives of Microbiology

, Volume 187, Issue 2, pp 87–99 | Cite as

A cryptic type I polyketide synthase (cpk) gene cluster in Streptomyces coelicolor A3(2)

  • Krzysztof Pawlik
  • Magdalena Kotowska
  • Keith F. Chater
  • Katarzyna Kuczek
  • Eriko Takano
Original Paper

Abstract

The chromosome of Streptomyces coelicolor A3(2), a model organism for the genus Streptomyces, contains a cryptic type I polyketide synthase (PKS) gene cluster which was revealed when the genome was sequenced. The ca. 54-kb cluster contains three large genes, cpkA, cpkB and cpkC, encoding the PKS subunits. Insilico analysis showed that the synthase consists of a loading module, five extension modules and a unique reductase as a terminal domain instead of a typical thioesterase. All acyltransferase domains are specific for a malonyl extender, and have a B-type ketoreductase. Tailoring and regulatory genes were also identified within the gene cluster. Surprisingly, some genes show high similarity to primary metabolite genes not commonly identified in any antibiotic biosynthesis cluster. Using western blot analysis with a PKS subunit (CpkC) antibody, CpkC was shown to be expressed in S. coelicolor at transition phase. Disruption of cpkC gave no obvious phenotype.

Keywords

Streptomyces Polyketide biosynthesis Post-polyketide modifications Antibiotic biosynthesis 

Abbreviations

AT

Acyltransferase

ACP

Acyl carrier protein

DH

Dehydratase

DEBS

6-Deoxyerythronolide synthase

ER

Enoyl reductase

KR

Ketoreductase

KS

β-Ketoacylsynthase

MS

Mannitol soya flour medium

NRPS

Non-ribosomal peptide synthetase

PKS

Polyketide synthase

SMM

Supplemented minimal medium

TD

Terminal reductase

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Copyright information

© Springer-Verlag 2006

Authors and Affiliations

  • Krzysztof Pawlik
    • 1
  • Magdalena Kotowska
    • 1
  • Keith F. Chater
    • 2
  • Katarzyna Kuczek
    • 1
  • Eriko Takano
    • 2
    • 3
  1. 1.Institute of Immunology and Experimental TherapyPolish Academy of SciencesWroclawPoland
  2. 2.John Innes Centre Norwich Research Park NorwichUK
  3. 3.Department of Microbial Physiology, Groningen Biomolecular Sciences and Biotechnology Institute (GBB)University of GroningenHarenThe Netherlands

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