Archives of Microbiology

, Volume 186, Issue 2, pp 107–118 | Cite as

Iron nutrition and physiological responses to iron stress in Nitrosomonas europaea

  • Xueming Wei
  • Neeraja Vajrala
  • Loren Hauser
  • Luis A. Sayavedra-Soto
  • Daniel J. Arp
Original Paper

Abstract

Nitrosomonas europaea, as an ammonia-oxidizing bacterium, has a high Fe requirement and has 90 genes dedicated to Fe acquisition. Under Fe-limiting conditions (0.2 μM Fe), N. europaea was able to assimilate up to 70% of the available Fe in the medium even though it is unable to produce siderophores. Addition of exogenous siderophores to Fe-limited medium increased growth (final cell mass). Fe-limited cells had lower heme and cellular Fe contents, reduced membrane layers, and lower NH3- and NH2OH-dependent O2 consumption activities than Fe-replete cells. Fe acquisition-related proteins, such as a number of TonB-dependent Fe-siderophore receptors for ferrichrome and enterobactin and diffusion protein OmpC, were expressed to higher levels under Fe limitation, providing biochemical evidence for adaptation of N. europaea to Fe-limited conditions.

Keywords

Nitrosomonas europaea Iron metabolism Iron stress Siderophore receptors 

Abbreviations

OM

Outer membrane

AMO

Ammonia monooxygenase

HAO

Hydroxylamine oxidoreductase

HPLC/MS/MS

High performance liquid chromatography tandem mass spectrometry

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Copyright information

© Springer-Verlag 2006

Authors and Affiliations

  • Xueming Wei
    • 1
  • Neeraja Vajrala
    • 1
  • Loren Hauser
    • 2
  • Luis A. Sayavedra-Soto
    • 1
  • Daniel J. Arp
    • 1
  1. 1.Department of Botany and Plant PathologyOregon State UniversityCorvallisUSA
  2. 2.Genome Science and Technology, Genome Analysis and Systems Modeling, Life Sciences Division, Oak Ridge National LaboratoryUniversity of TennesseeOak RidgeUSA

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