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Archives of Microbiology

, Volume 186, Issue 2, pp 107–118 | Cite as

Iron nutrition and physiological responses to iron stress in Nitrosomonas europaea

  • Xueming Wei
  • Neeraja Vajrala
  • Loren Hauser
  • Luis A. Sayavedra-Soto
  • Daniel J. Arp
Original Paper

Abstract

Nitrosomonas europaea, as an ammonia-oxidizing bacterium, has a high Fe requirement and has 90 genes dedicated to Fe acquisition. Under Fe-limiting conditions (0.2 μM Fe), N. europaea was able to assimilate up to 70% of the available Fe in the medium even though it is unable to produce siderophores. Addition of exogenous siderophores to Fe-limited medium increased growth (final cell mass). Fe-limited cells had lower heme and cellular Fe contents, reduced membrane layers, and lower NH3- and NH2OH-dependent O2 consumption activities than Fe-replete cells. Fe acquisition-related proteins, such as a number of TonB-dependent Fe-siderophore receptors for ferrichrome and enterobactin and diffusion protein OmpC, were expressed to higher levels under Fe limitation, providing biochemical evidence for adaptation of N. europaea to Fe-limited conditions.

Keywords

Nitrosomonas europaea Iron metabolism Iron stress Siderophore receptors 

Abbreviations

OM

Outer membrane

AMO

Ammonia monooxygenase

HAO

Hydroxylamine oxidoreductase

HPLC/MS/MS

High performance liquid chromatography tandem mass spectrometry

Notes

Acknowledgments

We thank Dr. B. Dubbels for his insightful discussions, B. Arbogast (Central Laboratory, OSU) for help with the HPLC/MS/MS analysis, and M. Nesson for help with electron microscopy (Electron Microscope Facility, Department of Botany and Plant Pathology, OSU). We appreciate the help of A. Ungerer (College of Oceanic and Atmospheric Sciences, OSU) for Fe determination by ICP-MS. This research was supported by grant DE-FG03-01ER63149 to DJA.

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Copyright information

© Springer-Verlag 2006

Authors and Affiliations

  • Xueming Wei
    • 1
  • Neeraja Vajrala
    • 1
  • Loren Hauser
    • 2
  • Luis A. Sayavedra-Soto
    • 1
  • Daniel J. Arp
    • 1
  1. 1.Department of Botany and Plant PathologyOregon State UniversityCorvallisUSA
  2. 2.Genome Science and Technology, Genome Analysis and Systems Modeling, Life Sciences Division, Oak Ridge National LaboratoryUniversity of TennesseeOak RidgeUSA

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