Archives of Microbiology

, Volume 185, Issue 2, pp 147–158 | Cite as

Molecular characterisation of ABC transporter type FtsE and FtsX proteins of Mycobacterium tuberculosis

  • Mushtaq Ahmad Mir
  • Haryadi S. Rajeswari
  • Usha Veeraraghavan
  • Parthasarathi Ajitkumar
Original Paper

Abstract

Elicitation of drug resistance and various survival strategies inside host macrophages have been the hallmarks of Mycobacterium tuberculosis as a successful pathogen. ATP Binding Cassette (ABC) transporter type proteins are known to be involved in the efflux of drugs in bacterial and mammalian systems. FtsE, an ABC transporter type protein, in association with the integral membrane protein FtsX, is involved in the assembly of potassium ion transport proteins and probably of cell division proteins as well, both of which being relevant to tubercle bacillus. In this study, we cloned ftsE gene of M. tuberculosis, overexpressed and purified. The recombinant MtFtsE-6xHis protein and the native MtFtsE protein were found localized on the membrane of E. coli and M. tuberculosis cells, respectively. MtFtsE-6xHis protein showed ATP binding in vitro, for which the K42 residue in the Walker A motif was found essential. While MtFtsE-6xHis protein could partially complement growth defect of E. coli ftsE temperature-sensitive strain MFT1181, co-expression of MtFtsE and MtFtsX efficiently complemented the growth defect, indicating that the MtFtsE and MtFtsX proteins might be performing an associated function. MtFtsE and MtFtsX-6xHis proteins were found to exist as a complex on the membrane of E. coli cells co-expressing the two proteins.

Keywords

FtsE FtsX ABC transporter type protein Walker A motif ATP binding Mycobacterium tuberculosis cell division 

Abbreviations

DTSP

3, 3′-dithio-bis(propionic acid N-hydroxysuccinimide ester)

ABC

ATP binding Cassette

DTT

Dithiothreitol

IPTG

Isopropylthio-β-d-galactopyranoside

CNBr

Cyanogen bromide

SRP

Signal Recognition Particle

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Copyright information

© Springer-Verlag 2006

Authors and Affiliations

  • Mushtaq Ahmad Mir
    • 1
  • Haryadi S. Rajeswari
    • 1
  • Usha Veeraraghavan
    • 1
  • Parthasarathi Ajitkumar
    • 1
  1. 1.Department of Microbiology and Cell BiologyIndian Institute of ScienceBangaloreIndia

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