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Archives of Microbiology

, Volume 184, Issue 3, pp 187–193 | Cite as

Production of volatile organic compounds (VOCs) by yeasts isolated from the ascocarps of black (Tuber melanosporum Vitt.) and white (Tuber magnatum Pico) truffles

  • Pietro Buzzini
  • Chiara Gasparetti
  • Benedetta Turchetti
  • Maria Rita Cramarossa
  • Ann Vaughan-Martini
  • Alessandro Martini
  • Ugo Maria Pagnoni
  • Luca Forti
Original Paper

Abstract

Twenty-nine yeast strains were isolated from the ascocarps of black and white truffles (Tuber melanosporum Vitt. and Tuber magnatum Pico, respectively), and identified using a polyphasic approach. According to the conventional taxonomic methods, MSP-PCR fingerprinting and sequencing of the D1/D2 domain of 26S rDNA, the strains were identified as Candida saitoana, Debaryomyces hansenii, Cryptococcus sp., Rhodotorula mucilaginosa, and Trichosporon moniliiforme. All isolates assimilated l-methionine as a sole nitrogen source and produced the volatile organic compounds (VOCs), 2-methyl butanol, 3-methyl butanol, methanethiol, S-methyl thioacetate, dimethyl sulfide, dimethyl disulfide, dimethyl trisulfide, dihydro-2-methyl-3(2H)-thiophenone and 3-(methylthio)-1-propanol (MTP). ANOVA analysis of data showed significant (P<0.01) differences in VOCs produced by different yeasts, with MTP as the major component (produced at concentrations ranging from 19.8 to 225.6 mg/l). In addition, since some molecules produced by the isolates of this study are also characteristic of truffle complex aroma, it is possible to hypothesize a complementary role of yeasts associated with this ecosystem in contributing to final Tuber spp. aroma through the independent synthesis of yeast-specific volatile constituents.

Keywords

VOC producing yeasts Black and white truffles Aroma Tubermelanosporum Vitt. Tubermagnatum Pico 

Notes

Acknowledgements

Authors are grateful to Prof. I. Spencer-Martins and to Dr. Gabriella Giménez Jurado of the Centro de Recursos Microbiológicos (CREM), Secção Autónoma de Biotecnologia, Faculdade de Ciências e Tecnologia, Universidade Nova de Lisboa (Portugal) for the their technical assistance in the course of molecular identification, and to M. Milito for her appreciable work in the course of this study.

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Copyright information

© Springer-Verlag 2005

Authors and Affiliations

  • Pietro Buzzini
    • 1
  • Chiara Gasparetti
    • 1
  • Benedetta Turchetti
    • 1
  • Maria Rita Cramarossa
    • 2
  • Ann Vaughan-Martini
    • 1
  • Alessandro Martini
    • 1
  • Ugo Maria Pagnoni
    • 2
  • Luca Forti
    • 2
  1. 1.Dipartimento di Biologia Vegetale e Biotecnologie Agroambientali, Sezione di Microbiologia ApplicataUniversità di PerugiaPerugiaItaly
  2. 2.Dipartimento di ChimicaUniversità di Modena e Reggio EmiliaModenaItaly

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