Archives of Microbiology

, Volume 184, Issue 1, pp 78–81 | Cite as

Differential expression and extent of fungal/plant and fungal/bacterial chitinases of Aspergillus fumigatus

  • Mariam Taib
  • John W. Pinney
  • David R. Westhead
  • Kenneth J. McDowall
  • David J. Adams
Short Communication

Abstract

We provide the first indication of the extent of the complex chitinolytic system of a filamentous fungus. Phylogenetic analysis of the 14 apparent chitinases of the opportunistic fungal pathogen Aspergillus fumigatus identified four and ten enzymes related to plant and bacterial chitinases, respectively. Further, real time-RT-PCR studies revealed distinct patterns of gene expression, consistent with morphogenetic or nutritional roles, for members of the fungal/plant or fungal/bacterial sub-families, respectively. Our results provide a basis for future studies with A. fumigatus chitinases, which may lead to the exploitation of these enzymes, or their regulators, in the development of novel drug strategies.

Keywords

Chitinase sub-families Aspergillus fumigatus Real time-RT-PCR Transcriptional regulation 

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Copyright information

© Springer-Verlag 2005

Authors and Affiliations

  • Mariam Taib
    • 1
  • John W. Pinney
    • 1
  • David R. Westhead
    • 1
  • Kenneth J. McDowall
    • 2
  • David J. Adams
    • 1
  1. 1.School of Biochemistry & MicrobiologyUniversity of LeedsLeedsUK
  2. 2.School of BiologyUniversity of LeedsLeedsUK

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