Archives of Microbiology

, Volume 183, Issue 1, pp 56–65

Chlorinated aliphatic hydrocarbon-induced degradation of trichloroethylene in Wautersia numadzuensis sp. nov.

  • Chizuko Kageyama
  • Toshiya Ohta
  • Kazuyo Hiraoka
  • Morimasa Suzuki
  • Tetsuji Okamoto
  • Kazuo Ohishi
Original Paper

DOI: 10.1007/s00203-004-0746-5

Cite this article as:
Kageyama, C., Ohta, T., Hiraoka, K. et al. Arch Microbiol (2005) 183: 56. doi:10.1007/s00203-004-0746-5

Abstract

Two strains of trichloroethylene (TCE)-degrading bacteria were isolated from soils at polluted and unpolluted sites. The isolates, strains TE26T and K6, showed co-substrate-independent TCE-degrading activity. TCE degradation was accelerated by preincubation with tetrachloroethylene, cis-dichloroethylene (DCE) and 1,1-DCE. TCE-degrading activities of strains TE26T and K6 were 0.23, 0.24 μmol min−1 g−1 dry cells, respectively. 16S rDNA sequences of strains TE26T and K6 were almost identical (99.7% similarity), and most closely related to Ralstonia basilensis (ATCC17697T) (98.5% similarity). From the results of DNA–DNA hybridizations, strain TE26T was genetically coherent to strain K6 (94 and 88% hybridization), and exhibited lower relatedness to R. basilensis (DSM11853T) (44% and 15%). In addition, because of the differences in chemotaxonomic properties, strain TE26T and strain K6 appear to be distinct from all established species of the Ralstonia group. Based on these results and the proposal of transferring R. basilensis and related species to Wautersia gen. nov., we propose that these strains should be assigned to the genus Wautersia as Wautersia numadzuensis sp. nov.

Keywords

Trichloroethylene Dichloroethylene Chlorinated aliphatic hydrocarbon Trichloroethylene degradation Bioremediation Wautersia numadzuensis sp. nov. 

Abbreviations

DCE

Dichloroethylene

LB

Luria-Bertani

OD

Optical density

PCE

Tetrachloroethylene

TCE

Trichloroethylene

Copyright information

© Springer-Verlag 2004

Authors and Affiliations

  • Chizuko Kageyama
    • 1
    • 2
  • Toshiya Ohta
    • 1
  • Kazuyo Hiraoka
    • 1
    • 3
  • Morimasa Suzuki
    • 1
    • 4
  • Tetsuji Okamoto
    • 1
    • 3
  • Kazuo Ohishi
    • 1
  1. 1.Numazu Industrial Research Institute of Shizuoka PrefectureShizuokaJapan
  2. 2.Shizuoka Agricultural experiment stationShizuokaJapan
  3. 3.Shizuoka Industrial Research Institute of Shizuoka PrefectureShizuoka-shiJapan
  4. 4.Living Environment Office, Shizuoka Prefectural GovernmentShizuoka CityJapan

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