Archives of Microbiology

, Volume 182, Issue 2–3, pp 259–263

Characterization of a heme oxygenase of Clostridium tetani and its possible role in oxygen tolerance

  • Holger Brüggemann
  • Rosalie Bauer
  • Stéphanie Raffestin
  • Gerhard Gottschalk
Short Communication

DOI: 10.1007/s00203-004-0721-1

Cite this article as:
Brüggemann, H., Bauer, R., Raffestin, S. et al. Arch Microbiol (2004) 182: 259. doi:10.1007/s00203-004-0721-1

Abstract

In order to colonize mammalian wounds, the anaerobic bacterium Clostridium tetani must presumably cope with temporary oxic conditions. Therefore, the recently decoded genome sequence was searched for genes which could confer oxygen tolerance. A few identified systems such as superoxide dismutases and peroxidases are probably responsible for this protection against toxic oxygen species. Another system was detected, a heme oxygenase which could have a role in establishing or maintaining an anoxic microenvironment in the process of wound colonization. The hemT gene encoding the heme oxygenase is expressed in C. tetani, as shown by reverse transcription–PCR. When overexpressed in Escherichia coli, the enzyme converts heme to biliverdin under strict oxic conditions.

Keywords

Clostridium tetani Tetanus Heme oxygenase Oxidative stress Aerotolerance Iron acquisition 

Copyright information

© Springer-Verlag 2004

Authors and Affiliations

  • Holger Brüggemann
    • 1
    • 2
  • Rosalie Bauer
    • 1
    • 3
  • Stéphanie Raffestin
    • 4
  • Gerhard Gottschalk
    • 1
  1. 1.Göttingen Genomics Laboratory, Institute of Microbiology and GeneticsGeorg-August-University GöttingenGöttingenGermany
  2. 2.Laboratoire de Génomique des Microorganismes PathogènesInstitut PasteurParisFrance
  3. 3.Departments of Microbiology and ParasitologyUmweltbundesamtBerlinGermany
  4. 4.Unité des Bactéries Anaérobies et ToxinesInstitut PasteurParisFrance

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