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Archives of Microbiology

, Volume 182, Issue 2–3, pp 147–156 | Cite as

Microorganisms degrading chlorobenzene via a meta-cleavage pathway harbor highly similar chlorocatechol 2,3-dioxygenase-encoding gene clusters

  • Markus Göbel
  • Oliver H. Kranz
  • Stefan R. Kaschabek
  • Eberhard Schmidt
  • Dietmar H. Pieper
  • Walter ReinekeEmail author
Original Paper

Abstract

Pseudomonas putida GJ31 harbors a degradative pathway for chlorobenzene via meta-cleavage of 3-chlorocatechol. Pseudomonads using this route for chlorobenzene degradation, which was previously thought to be generally unproductive, were isolated from various contaminated environments of distant locations. The new isolates, Pseudomonas fluorescens SK1 (DSM16274), Pseudomonas veronii 16-6A (DSM16273), Pseudomonas sp. strain MG61 (DSM16272), harbor a chlorocatechol 2,3-dioxygenase (CbzE). The cbzE-like genes were cloned, sequenced, and expressed from the isolates and a mixed culture. The chlorocatechol 2,3-dioxygenases shared 97% identical amino acids with CbzE from strain GJ31, forming a distinct family of catechol 2,3-dioxygenases. The chlorocatechol 2,3-dioxygenase, purified from chlorobenzene-grown cells of strain SK1, showed an identical N-terminal sequence with the amino acid sequence deduced from cloned cbzE. In all investigated chlorobenzene-degrading strains, cbzT-like genes encoding ferredoxins are located upstream of cbzE. The sequence data indicate that the ferredoxins are identical (one amino acid difference in CbzT of strain 16-6A compared to the others). In addition, the structure of the operon downstream of cbzE is identical in strains GJ31, 16-6A, and SK1 with genes cbzX (unknown function) and the known part of cbzG (2-hydroxymuconic semialdehyde dehydrogenase) and share 100% nucleotide sequence identity with the entire downstream region. The current study suggests that meta-cleavage of 3-chlorocatechol is not an atypical pathway for the degradation of chlorobenzene.

Keywords

Degradation of chloroaromatic compounds Meta-cleavage pathway 

Notes

Acknowledgments

This work was financed by a grant from the Deutsche Forschungsgemeinschaft (Re659/7-1) and by the European Union, contract EVK1-CT1999-00023 “MAROC”. The MAROC samples were supplied by Bioclear, Groningen, The Netherlands. We thank Y. Jouanneau, Grenoble, France, for critical reading of the manuscript, U. Pagga, BASF, Ludwigshafen, Germany, for a waste water sample, N.C. McClure, Flinders University of South Australia, Adelaide, Australia, for sending pTDN1-1018, and V. Nödinger, University Stuttgart, Germany, for the amino acid sequencing. We are indebted to CEA for providing the environment in which O.K. could do his work in Grenoble.

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Copyright information

© Springer-Verlag 2004

Authors and Affiliations

  • Markus Göbel
    • 1
    • 2
  • Oliver H. Kranz
    • 1
  • Stefan R. Kaschabek
    • 3
  • Eberhard Schmidt
    • 1
  • Dietmar H. Pieper
    • 4
  • Walter Reineke
    • 1
    Email author
  1. 1.Chemische MikrobiologieBergische Universität WuppertalWuppertalGermany
  2. 2.Department of BiologyThe University of KonstanzKonstanzGermany
  3. 3.Interdisziplinäres Ökologisches ZentrumTU Bergakademie FreibergFreibergGermany
  4. 4.Department o f Environmental MicrobiologyGBF-German Research Center for BiotechnologyBraunschweigGermany

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