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Archives of Microbiology

, Volume 181, Issue 1, pp 17–25 | Cite as

Phylogenetic and physiological characterization of a filamentous anoxygenic photoautotrophic bacterium ‘Candidatus Chlorothrix halophila’ gen. nov., sp. nov., recovered from hypersaline microbial mats

  • Joel A. Klappenbach
  • Beverly K. Pierson
Original Paper

Abstract

We report the phylogenetic and physiological characterization of a mesophilic and halophilic member of the filamentous anoxygenic phototrophic (FAP) bacteria, provisionally named ‘Candidatus Chorothrix halophila’ gen. nov. sp. nov., that has been maintained in a highly enriched culture in our laboratory for over a decade. Phylogenetic analysis of small-subunit RNA-encoding sequences places ‘Candidatus Chlorothrix halophila’ in a clade that includes cultivated members of the genera Chloroflexus and Oscillochloris. Physiological studies demonstrated sulfide-dependent photosynthetic uptake of 14C-labeled bicarbonate. Enzymatic assays for the activity of propionyl-coenzyme A synthase indicated that ‘Candidatus Chlorothrix halophila’ does not use the 3-hydroxypropionate cycle of Chloroflexus aurantiacus OK-70-fl for autotrophic carbon assimilation. New concepts regarding the taxonomy and phylogeny of FAP bacteria have emerged from this work.

Keywords

Green non-sulfur 3-Hydroxypropionate Candidatus Chlorothrix halophila’ Marine Chloroflexus-like organisms 

Abbreviations

MCLO

Marine Chloroflexus-like organism

FAP

Filamentous anoxygenic phototroph

Notes

Acknowledgements

This work was supported by a NASA Astrobiology grant to Arizona State University with a sub-contract to BKP at the University of Puget Sound. We thank M.N. Parenteau, M. Lawrence, E. Morgan and E. Lilja for technical assistance; T.M. Schmidt for advice and use of laboratory equipment; and, the laboratory of N.R. Pace for preliminary work on phylogenetic identification.

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Copyright information

© Springer-Verlag 2004

Authors and Affiliations

  1. 1.Center for Microbial Ecology and Department of Microbiology and Molecular GeneticsMichigan State UniversityEast LansingUSA
  2. 2.Department of BiologyUniversity of Puget SoundTacomaUSA

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