Archives of Microbiology

, Volume 180, Issue 6, pp 444–454

Diverse Mesorhizobium plurifarium populations native to Mexican soils

  • En Tao Wang
  • Feng Ling Kan
  • Zhi Yuan Tan
  • Ivonne Toledo
  • Wen Xin Chen
  • Esperanza Martínez-Romero
Original Paper

Abstract

Forty-six Mesorhizobium strains associated with the leguminous plants Leucaena leucocephala and Sesbania herbacea in an uncultivated Mexican field were characterized using a polyphasic approach. The strains were identified as Mesorhizobium plurifarium based upon the close relationships with the reference strains for this species in PCR-based restriction fragment length polymorphism analyses, sequencing of 16S rRNA genes, multilocus enzyme electrophoresis, and DNA-DNA hybridization. Although the strains isolated from both plants formed the same group in multilocus enzyme electrophoresis and cross-nodulations were observed in the laboratory, different electrophoretic types were obtained from the two plants grown in natural soils, indicating the existence of a preferable association between the plants and the rhizobia. The M. plurifarium strains from Mexico and the reference strains from Africa and Brazil formed different phenotypic clusters in a numerical taxonomy. The Mexican strains did not grow at 37 °C and were sensitive to salty-alkaline conditions, while the reference strains from Africa and Brazil grew at 42 °C and were more resistant to salty-alkaline conditions. These results demonstrate that both the plants and environmental factors affected the evolution of rhizobia and that the Mexican strains had adapted to the neutral soils and the cool climate where they were isolated.

Keywords

Mesorhizobium plurifarium Leucaena Sesbania Phylogeny Diversity 

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Copyright information

© Springer-Verlag 2003

Authors and Affiliations

  • En Tao Wang
    • 1
    • 4
  • Feng Ling Kan
    • 2
  • Zhi Yuan Tan
    • 3
  • Ivonne Toledo
    • 4
  • Wen Xin Chen
    • 2
  • Esperanza Martínez-Romero
    • 4
  1. 1.Departamento de MicrobiologíaInstituto Politécnico NacionalCarpio y Plan de Ayala S/NMéxico
  2. 2.Department of MicrobiologyChina Agricultural UniversityBeijingP.R. China
  3. 3.Department of Molecular GeneticsSouth China Agricultural UniversityGuangzhouChina
  4. 4.Centro de Investigación sobre Fijación de NitrógenoUniversidad Nacional Autonoma de MéxicoCuernavacaMéxico

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