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Archives of Microbiology

, Volume 180, Issue 5, pp 339–346 | Cite as

Chromosomally encoded gyrase inhibitor GyrI protects Escherichia coli against DNA-damaging agents

  • Monalisa Chatterji
  • Sugopa Sengupta
  • Valakunja NagarajaEmail author
Article

Abstract

DNA gyrase, a type II topoisomerase, is the sole supercoiling activity in the cell and is essential for cell survival. There are two proteinaceous inhibitors of DNA gyrase that are plasmid-borne and ensure maintenance of the plasmids in bacterial populations. However, the physiological role of GyrI, an inhibitor of DNA gyrase encoded by the Escherichia coli genome, has been elusive. Previously, we have shown that GyrI imparts resistance against microcin B17 and CcdB. Here, we find that GyrI provided partial/limited protection against the quinolone class of gyrase inhibitors but had no effect on inhibitors that interfere with the ATPase activity of the enzyme. Moreover, GyrI negated the effect of alkylating agents, such as mitomycin C and N-methyl-N-nitro-N-nitrosoguanidine, that act independently of DNA gyrase. Hence, in vivo, GyrI appears to be involved in reducing DNA damage from many sources. In contrast, GyrI is not effective against lesions induced by ultraviolet radiation. Furthermore, the expression of GyrI does not significantly alter the topology of DNA. Thus, although isolated as an inhibitor of DNA gyrase, GyrI seems to have a broader role in vivo than previously envisaged.

Keywords

DNA gyrase Microcin B17 GyrI SbmC CcdB 

Notes

Acknowledgements

We thank A. Piekarowicz and D.C. Stein for E. coli strain AP1-200-9. We also thank S. Unniraman for invaluable suggestions and Chandrima Das for technical help. This work was supported by the Department of Science and Technology, Government of India.

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Copyright information

© Springer-Verlag 2003

Authors and Affiliations

  • Monalisa Chatterji
    • 1
    • 3
  • Sugopa Sengupta
    • 1
  • Valakunja Nagaraja
    • 1
    • 2
    Email author
  1. 1.Department of Microbiology and Cell BiologyIndian Institute of ScienceBangaloreIndia
  2. 2.Jawaharlal Nehru Centre for Advanced Scientific ResearchBangaloreIndia
  3. 3.HHMI/Section of ImmunobiologyYale University Medical SchoolNew HavenUSA

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