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Archives of Microbiology

, Volume 180, Issue 5, pp 327–338 | Cite as

Isolation and characterization of Erythrobacter sp. strains from the upper ocean

  • Michal Koblížek
  • Oded Béjà
  • Robert R. Bidigare
  • Stephanie Christensen
  • Bryan Benitez-Nelson
  • Costantino Vetriani
  • Marcin K. Kolber
  • Paul G. Falkowski
  • Zbigniew S. Kolber
Original Paper

Abstract

Seven strains of marine aerobic anoxygenic phototrophs belonging to the genus Erythrobacter were isolated. The strains were characterized regarding their physiological and biochemical properties, 16S rDNA and pufM gene sequences, morphological features, substrate preference, as well as pigment and lipid composition. All strains had functional type-2 reaction centers containing bacteriochlorophyll, served by small, light-harvesting complex 1, and were photosynthetically competent. In addition, large pools of carotenoids were found, but only some of the accessory pigments transfer energy to the reaction centers. All of the isolates were facultative photoheterotrophs. They required an organic carbon substrate for growth; however, they are able to supplement a significant fraction of their metabolic requirements with photosynthetically derived energy.

Keywords

Aerobic anoxygenic phototrophs Aerobic photosynthetic bacteria Bacteriochlorophyll a Erythrobacter Photoheterotrophy 

Abbreviations

BChl

Bacteriochlorophyll

Chl

Chlorophyll

Erb.

Erythrobacter

Erm.

Erythromicrobium

FAMEs

Fatty acid methyl esters

IRFRR

Infrared fast repetition rate

LH1, LH2

Light-harvesting complex 1 and 2, respectively

Por.

Porphyrobacter

PUFAs

Polyunsaturated fatty acids

Rsb.

Roseobacter

RubisCO

Ribulose-1,5-bisphosphate carboxylase/oxygenase

µ

Growth rate

σ470

Functional cross-section of the photosynthetic unit at 470 nm

Notes

Acknowledgements

The authors thank Maxim Gorbunov, Michael Behrenfeld, Yoram Gerchman and Ondrej Prasil for supplying the water samples, and Kevin Wyman for laboratory assistance. This research was supported by Rutgers University through a Post-doctoral Research Fellowship to MK and by grants from NSF (Biocomplexity to PGF, and OCE-022095 to ZSK), EEC-9731725 (RRB) and OCE-9617409 (RRB), from NASA NAG5-7171 (RRB) and NAGW-3439 (RRB) and from Czech MSMT projects LN00A141 and MSM12310001 (MK) and GACR 206/03/P079 (MK).

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Copyright information

© Springer-Verlag 2003

Authors and Affiliations

  • Michal Koblížek
    • 1
    • 6
  • Oded Béjà
    • 2
  • Robert R. Bidigare
    • 3
  • Stephanie Christensen
    • 3
  • Bryan Benitez-Nelson
    • 3
  • Costantino Vetriani
    • 4
  • Marcin K. Kolber
    • 1
  • Paul G. Falkowski
    • 1
    • 5
  • Zbigniew S. Kolber
    • 1
    • 7
  1. 1.Environmental Biophysics and Molecular Ecology ProgramRutgers UniversityNew BrunswickUSA
  2. 2.Department of BiologyTechnion-Israel Institute of TechnologyHaifaIsrael
  3. 3.Department of OceanographyUniversity of Hawai'i at ManoaHonoluluUSA
  4. 4.Department of Biochemistry and MicrobiologyRutgers UniversityNew BrunswickUSA
  5. 5.Department of GeologyRutgers UniversityPiscatawayUSA
  6. 6.Institute of MicrobiologyTřeboňCzechia
  7. 7.MBARIUSA

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