A transporter of Escherichia coli specific for l- and d-methionine is the prototype for a new family within the ABC superfamily
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An ABC-type transporter in Escherichia coli that transports both l- and d-methionine, but not other natural amino acids, was identified. This system is the first functionally characterized member of a novel family of bacterial permeases within the ABC superfamily. This family was designated the methionine uptake transporter (MUT) family (TC #3.A.1.23). The proteins that comprise the transporters of this family were analyzed phylogenetically, revealing the probable existence of several sequence-divergent primordial paralogues, no more than two of which have been transmitted to any currently sequenced organism. In addition, MetJ, the pleiotropic methionine repressor protein, was shown to negatively control expression of the operon encoding the ABC-type methionine uptake system. The identification of MetJ binding sites (in gram-negative bacteria) or S-boxes (in gram-positive bacteria) in the promoter regions of several MUT transporter-encoding operons suggests that many MUT family members transport organic sulfur compounds.
KeywordsTransport Methionine MetD ATP-binding cassette E. coli
This work was supported by NIH grants GM55434 and GM64368 from the National Institute of General Medical Sciences (to MHS). JNF was supported by a Rhùne-Alpes Fellowship from France. We thank Mary Beth Hiller for her assistance in the preparation of this manuscript.
- Greene RC (1996) Biosynthesis of methionine. In Neidhardt FC (ed) Escherichia coli and Salmonella: cellular and molecular miology, 2nd edn, vol 1. ASM Press, Washington DC, pp. 542–560Google Scholar
- Sambrook J, Fritsch EF, Maniatis T (1989) Molecular cloning: a laboratory manual, 2nd edn. Cold Spring Harbor Laboratory, Cold Spring Harbor, New YorkGoogle Scholar
- Zhang Z, Aboulwafa M, Smith M, Saier MH Jr. (2003) The ascorbate transporter of Escherichia coli. J Bacteriol 185:2243–2250Google Scholar