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Archives of Microbiology

, Volume 179, Issue 6, pp 394–401 | Cite as

Palaeococcus helgesonii sp. nov., a facultatively anaerobic, hyperthermophilic archaeon from a geothermal well on Vulcano Island, Italy

  • Jan P. AmendEmail author
  • D'Arcy R. Meyer-Dombard
  • Seema N. Sheth
  • Natalya Zolotova
  • Andrea C. Amend
Original Paper

Abstract

A novel, hyperthermophilic archaeon was isolated from a shallow geothermal well that taps marine waters on the Island of Vulcano in the southern Tyrrhenian Sea, Italy. The cells were irregular cocci, 0.6–1.5 μm in diameter, with multiple polar flagella. Growth was observed at temperatures from 45 to 85 °C (optimum at ~80 °C), pH 5–8 (optimum at 6.5), and 0.5–6.0% NaCl (optimum at ~2.8%). The minimum doubling time was 50 min. The isolate was obligately chemoheterotrophic, utilizing complex organic compounds including yeast or beef extract, peptone, tryptone, or casein for best growth. The presence of elemental sulfur enhanced growth. The isolate grew anaerobically as well as microaerobically. The G+C content of the genomic DNA was 42.5 mol%. The 16S rRNA sequence indicated that the new isolate was a member of the Thermococcales within the euryarchaeota, representing the second species in the genus Palaeococcus. Its physiology and phylogeny differed in several key characteristics from those of Palaeococcus ferrophilus, justifying the establishment of a new species; the name Palaeococcus helgesonii sp. nov. is proposed, type strain PI1 (DSM 15127).

Keywords

Palaeococcus Hyperthermophilic archaeon Shallow marine hydrothermal system Facultative anaerobe Microaerophilic growth 

Notes

Acknowledgements

We thank Melanie Holland for assistance in constructing the phylogenetic tree and for providing the sequence of the 23S primer 64Ra designed by her. Help with electron and light microscopy from Sherry Cady and Niki Parenteau at Portland State University and Wandy Beatty at the Molecular Microbiology Imaging Facility at Washington University is greatly appreciated. This work benefited from field assistance by and discussions with Everett Shock, Sergio Gurrieri, Salvo Inguaggiato, Franco Italiano, and Toti Francofonte. JPA would like to thank Hal Helgeson for introducing him to the scientific opportunities afforded by the hydrothermal system of the Aeolian Islands, but more importantly, for introducing him to Mariano Valenza, Mario Nuccio, and Sergio Gurrieri in Palermo, without whom none of this work could have been started, let alone come to fruition. Financial support was provided by NSF Grants OCE-9714288 and OCE-0221417 to JPA and NASA-GSRP grant NGT5-50348 to DRM-D.

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Copyright information

© Springer-Verlag 2003

Authors and Affiliations

  • Jan P. Amend
    • 1
    • 2
    Email author
  • D'Arcy R. Meyer-Dombard
    • 1
  • Seema N. Sheth
    • 1
  • Natalya Zolotova
    • 1
  • Andrea C. Amend
    • 1
  1. 1.Department of Earth and Planetary Sciences and Division of Biology and Biomedical SciencesWashington UniversitySt. LouisUSA
  2. 2.Department of Earth and Planetary SciencesWashington UniversitySt. LouisUSA

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