Archives of Microbiology

, Volume 179, Issue 4, pp 258–265

Characterization of a new keratinolytic bacterium that completely degrades native feather keratin

  • Alessandro Riffel
  • Françoise Lucas
  • Philipp Heeb
  • Adriano Brandelli
Original Paper
  • 1.5k Downloads

Abstract

A novel feather-degrading microorganism was isolated from poultry waste, producing a high keratinolytic activity when cultured on broth containing native feather. Complete feather degradation was achieved during cultivation. The bacterium presents potential use for biotechnological processes involving keratin hydrolysis. Chryseobacterium sp. strain kr6 was identified based on morphological and biochemical tests and 16S rRNA sequencing. The bacterium presented optimum growth at pH 8.0 and 30 °C; under these conditions, maximum feather-degrading activity was also achieved. Maximum keratinase production was reached at 25 °C, while concentration of soluble protein was similar at both 25 and 30 °C. Reduction of disulfide bridges was also observed, increasing with cultivation time. The keratinase of strain kr6 was active on azokeratin and azocasein as substrates, and presented optimum pH and temperature of 7.5 and 55 °C, respectively. The keratinase activity was inhibited by 1,10-phenanthroline, EDTA, Hg2+, and Cu2+ and stimulated by Ca2+.

Keywords

Keratin Proteolysis Poultry waste Chicken feather Bacteria 

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Copyright information

© Springer-Verlag 2003

Authors and Affiliations

  • Alessandro Riffel
    • 1
  • Françoise Lucas
    • 2
  • Philipp Heeb
    • 2
  • Adriano Brandelli
    • 1
  1. 1.Departamento de Ciência de Alimentos, ICTAUniversidade Federal do Rio Grande do SulPorto AlegreBrazil
  2. 2.Institut d'EcologieUniversity of LausanneLausanneSwitzerland

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