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Archives of Microbiology

, Volume 178, Issue 6, pp 477–483 | Cite as

Different effectors of dimorphism in Yarrowia lipolytica

  • José Ruiz-Herrera
  • Rafael Sentandreu
Original Paper

Abstract.

Yarrowia lipolytica is an ascomycete with biotechnological potential. In common media, the fungus grows as a mixture of yeast-like and short mycelial cells. The environmental factors that affect dimorphism in the wild-type strain, W29, and its auxotrophic derivative, PO1a, were analyzed. In both strains, pH was the most important factor regulating the dimorphic transition. Mycelium formation was maximal at pH near neutrality and decreased as pH was lowered to become almost null at pH 3. Carbon and nitrogen sources, namely glucose and ammonium, were also important for mycelium formation; and their effect was antagonized by some alternative carbon and nitrogen sources. Citrate was an important positive effector of mycelium growth. Anaerobic stress induced formation of mycelial cells. The importance of the protein kinase A pathway was suggested by the inhibition of mycelium growth by cAMP. We propose that the interplay of these factors regulates the adaptation of the fungus, to better exploit its natural ecological niches.

Dimorphic fungi Mycelial cells Yeast–mycelial cell transition cAMP 

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Copyright information

© Springer-Verlag 2002

Authors and Affiliations

  • José Ruiz-Herrera
    • 2
  • Rafael Sentandreu
    • 1
  1. 1.Sección de Microbiología, Facultad de Farmacia, Universidad de Valencia, Avenida Vicent Andrés Estellés s/n, 46100 Burjassot, València, Spain
  2. 2.Departamento de Ingeniería Genética, Unidad Irapuato, Centro de Investigación y de Estudios Avanzados del IPN, Irapuato, Guanajuato, Mexico

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