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Osteoporosis International

, Volume 29, Issue 8, pp 1917–1926 | Cite as

Vitamin D3 contributes to enhanced osteogenic differentiation of MSCs under oxidative stress condition via activating the endogenous antioxidant system

  • J. ZhouEmail author
  • F. Wang
  • Y. Ma
  • F. Wei
Original Article

Abstract

Summary

The anti-oxidative effects of vitamin D3 (Vd3) on mesenchymal stem cells (MSCs) have not been studied before. The present study suggested that Vd3 could not only promote the osteogenic differentiation of MSCs under normal condition but also partly protect it from oxidative stress damage by activating the endogenous antioxidant system.

Introduction

Evolving evidence proved that oxidative stress caused by reactive oxygen species (ROS) overproduction might lead to bone loss. Vd3, a commonly used osteogenic induction drug, was proved to exhibit potent anti-oxidative effects on other cell types. The present study aims to investigate the protective effects of Vd3 on oxidative stress-induced dysfunctions of MSCs, as well as its underlying mechanisms.

Methods

The H2O2 was used as exogenous reactive oxygen species (ROS). The influence of ROS and anti-oxidative protection of Vd3 on MSCs were analyzed too. Multi-techniques were used to assess the beneficial effects of Vd3 on MSCs under oxidative stress condition.

Results

The results demonstrated that Vd3 could significantly attenuate the H2O2-induced cell injury of MSCs via Sirt1/FoxO1 signaling pathway, and reduced the H2O2 exposure-induced intracellular oxidative stress status of MSCs. What’s more, the H2O2 exposure resulted in the decreased osteogenic differentiation of MSCs, as evidenced by decreased alkaline phosphatase activity, calcium deposition level, and osteogenic differentiation gene mRNA levels, but the injury was restored via Vd3 administration.

Conclusions

The results suggested that Vd3 could not only promote the osteogenic differentiation of osteoblastic cells under normal condition but also partly protect the cell from oxidative stress damage by activating endogenous antioxidant system. The study shed light on the new roles of Vd3 in bone modeling and remodeling regulation.

Keywords

Osteogenic differentiation Oxidative stress Protective effects Vitamin D3 

Notes

Compliance with ethical standards

Conflicts of interest

None.

Supplementary material

198_2018_4547_MOESM1_ESM.docx (5.4 mb)
ESM 1 (DOCX 5546 kb)

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Copyright information

© International Osteoporosis Foundation and National Osteoporosis Foundation 2018

Authors and Affiliations

  1. 1.Department of Orthopedics, Beijing Tongren HospitalCapital Medical UniversityBeijingPeople’s Republic of China

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