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Osteoporosis International

, Volume 28, Issue 3, pp 1133–1143 | Cite as

The development of inter-strain variation in cortical and trabecular traits during growth of the mouse lumbar vertebral body

  • M. A. Ramcharan
  • M. E. Faillace
  • Z. Guengerich
  • V. A. Williams
  • K. J. Jepsen
Original Article
  • 160 Downloads

Abstract

Summary

How cortical and trabecular bone co-develop to establish a mechanically functional structure is not well understood. Comparing early postnatal differences in morphology of lumbar vertebral bodies for three inbred mouse strains identified coordinated changes within and between cortical and trabecular traits. These early coordinate changes defined the phenotypic differences among the inbred mouse strains.

Introduction

Age-related changes in cortical and trabecular traits have been well studied; however, very little is known about how these bone tissues co-develop from day 1 of postnatal growth to establish functional structures by adulthood. In this study, we aimed to establish how cortical and trabecular tissues within the lumbar vertebral body change during growth for three inbred mouse strains that express wide variation in adult bone structure and function.

Methods

Bone traits were quantified for lumbar vertebral bodies of female A/J, C57BL/6J (B6), and C3H/HeJ (C3H) inbred mouse strains from 1 to 105 days of age (n = 6–10 mice/age/strain).

Results

Inter-strain differences in external bone size were observed as early as 1 day of age. Reciprocal and rapid changes in the trabecular bone volume fraction and alignment in the direction of axial compression were observed by 7 days of age. Importantly, the inter-strain difference in adult trabecular bone volume fraction was established by 7 days of age. Early variation in external bone size and trabecular architecture was followed by progressive increases in cortical area between 28 and 105 days of age, with the greatest increases in cortical area seen in the mouse strain with the lowest trabecular mass.

Conclusion

Establishing the temporal changes in bone morphology for three inbred mouse strains revealed that genetic variation in adult trabecular traits were established early in postnatal development. Early variation in trabecular architecture preceded strain-specific increases in cortical area and changes in cortical thickness. This study established the sequence of how cortical and trabecular traits co-develop during growth, which is important for identifying critical early ages to further focus on intervention studies that optimize adult bone strength.

Keywords

Bone histomorphometry Cortical Genetic variation Growth Trabecular 

Notes

Acknowledgments

We thank the National Institute of Health, National Institute of Arthritis and Musculoskeletal and Skin Diseases (AR056639, AR44927), for the support of this research. The content is solely the responsibility of the authors and does not necessarily represent the official views of the National Institute of Health.

Compliance with ethical standards

Conflicts of interest

None.

Supplementary material

198_2016_3801_MOESM1_ESM.docx (36 kb)
ESM 1 (DOCX 35 kb)
198_2016_3801_Fig6_ESM.gif (116 kb)
Supplemental Figure 1

Representative mid cross-sectional images of a mouse lumbar vertebrae indicating analysis of cortical and trabecular traits in the vertebral body. In the (A) mid-transverse plane, the outermost outline indicates cross-sectional total area (Tt.Ar). Cortical area (Ct.Ar) was calculated as Tt.Ar – Med.Ar (medullary area), where Med.Ar = marrow area + trabecular bone area. In the (B) mid-coronal plane, the outline indicates the area of interest between cranial and caudal growth plates used to determine the percentage of trabecular bone area (%Tb.Ar) and the average trabecular thickness (Tb.Th). (GIF 115 kb)

198_2016_3801_MOESM2_ESM.tif (4.1 mb)
High resolution image (TIF 4181 kb)

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Copyright information

© International Osteoporosis Foundation and National Osteoporosis Foundation 2016

Authors and Affiliations

  • M. A. Ramcharan
    • 1
    • 2
    • 3
  • M. E. Faillace
    • 2
  • Z. Guengerich
    • 2
  • V. A. Williams
    • 2
  • K. J. Jepsen
    • 3
  1. 1.Department of Biomedical EngineeringThe City College of New York c/o CUNY Graduate CenterNew YorkUSA
  2. 2.Leni and Peter W. May Department of OrthopaedicsIcahn School of Medicine at Mount SinaiNew YorkUSA
  3. 3.Department of Orthopaedic SurgeryUniversity of MichiganAnn ArborUSA

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