Effect of green tea and Tai Chi on bone health in postmenopausal osteopenic women: a 6-month randomized placebo-controlled trial
Postmenopausal women with osteopenia received green tea polyphenols (GTP) supplement and/or Tai Chi exercise for 6 months. Bone turnover biomarkers, calcium metabolism, and muscle strength were measured. This study showed that GTP supplementation and Tai Chi exercise increased bone formation biomarkers and improved bone turnover rate. Tai Chi exercise increased serum parathyroid hormone. GTP supplementation, Tai Chi exercise, and the combination of the two all improved muscle strength in postmenopausal women with osteopenia.
This study evaluated the effect of GTP supplementation and Tai Chi (TC) exercise on serum markers of bone turnover (bone-specific alkaline phosphatase, BAP, and tartrate-resistant acid phosphatase, TRAP), calcium metabolism, and muscle strength in postmenopausal osteopenic women.
One hundred and seventy-one postmenopausal osteopenic women were randomly assigned to four groups: (1) placebo (500 mg starch/day), (2) GTP (500 mg GTP/day), (3) placebo + TC (placebo plus TC training at 60 min/session, three sessions/week), and (4) GTP + TC (GTP plus TC training). Overnight fasting blood and urine samples were collected at baseline, 1, 3, and 6 months for biomarker analyses. Muscle strength was evaluated at baseline, 3, and 6 months. One hundred and fifty subjects completed the 6-month study.
Significant increases in BAP level due to GTP intake (at 1 month) and TC (at 3 months) were observed. Significant increases in the change of BAP/TRAP ratio due to GTP (at 3 months) and TC (at 6 months) were also observed. Significant main effect of TC on the elevation in serum parathyroid hormone level was observed at 1 and 3 months. At 6 months, muscle strength significantly improved due to GTP, TC, and GTP + TC interventions. Neither GTP nor TC affected serum TRAP, serum and urinary calcium, and inorganic phosphate.
In summary, GTP supplementation and TC exercise increased BAP and improved BAP/TRAP ratio. TC exercise increased serum parathyroid hormone. GTP supplementation, TC exercise, and the combination of the two all improved muscle strength in postmenopausal women with osteopenia.
KeywordsBone turnover biomarker Calcium metabolism Green tea Mind–body exercise Muscle strength Osteoporosis Tai Chi
This study was supported by the National Center for Complementary and Alternative Medicine (NCCAM) of the National Institutes of Health under grant 1R21AT003735-01A1. The contents of this manuscript are solely the responsibility of the authors and do not necessarily represent the official views of the NCCAM or the National Institutes of Health. We gratefully acknowledge the study participants; without them, this study would not have been possible. We would like to thank Dr. Jay Magaziner (University of Maryland, MD) for their advice. We also thank Mary J. Flores, Raul Y. Dagda, and Marisela Dagda for their assistance in data collection. Clinical trial number: NCT00625391.
Conflicts of interest
- 3.Hamdi Kara I, Aydin S, Gemalmaz A, Aktürk Z, Yaman H, Bozdemir N, Kurdak H, Sitmapinar K, Devran Sencar I, Basak O, Akdeniz M, Isildar H, Burgut E, Ozcan S, Akca U, Dağdeviren N, Ungagn M (2007) Habitual tea drinking and bone mineral density in postmenopausal Turkish women: investigation of prevalence of postmenopausal osteoporosis in Turkey (IPPOT Study). Int J Vitam Nutr Res 77(6):389–397, Erratum in: Int J Vitam Nutr Res 2008;78(3):following 166PubMedCrossRefGoogle Scholar
- 10.Kanis J, Johnell O, Gullberg B, Allander E, Elffors L, Ranstam J, Dequeker J, Dilsen G, Gennari C, Vaz AL, Lyritis G, Mazzuoli G, Miravet L, Passeri M, Perez Cano R, Rapado A, Ribot C (1999) Risk factors for hip fracture in men from southern Europe: the MEDOS Study. Mediterranean Osteoporosis Study. Osteoporos Int 9:45–54PubMedCrossRefGoogle Scholar
- 14.Vestergaard P, Hermann AP, Gram J, Jensen LB, Eiken P, Abrahamsen B, Brot C, Kolthoff N, Sørensen OH, Beck Nielsen H, Pors Nielsen S, Charles P, Mosekilde L (2001) Evaluation of methods for prediction of bone mineral density by clinical and biochemical variables in perimenopausal women. Maturitas 40(3):211–220PubMedCrossRefGoogle Scholar
- 17.Kasamatsu T, Yoshimura N, Morioka S, Sugita K, Hashimoto T (1996) A population survey on bone mineral density in a fishing village in Wakayama prefecture. (Part 1) Distribution of bone mineral density by sex and age based on a representative sample of the community. Nippon Eiseigaku Zasshi 50(6):1084–1092 [Japanese]PubMedCrossRefGoogle Scholar
- 20.Hamdi Kara I, Aydin S, Gemalmaz A, Aktürk Z, Yaman H, Bozdemir N, Kurdak H, Sitmapinar K, Devran Sencar I, Başak O, Akdeniz M, Işildar H, Burgut E, Ozcan S, Akça U, Dağdeviren N, Ungan M (2007) Habitual tea drinking and bone mineral density in postmenopausal Turkish women: investigation of prevalence of postmenopausal osteoporosis in Turkey (IPPOT Study). Int J Vitam Nutr Res 77(6):389–397PubMedCrossRefGoogle Scholar
- 23.Dorchies OM, Wagner S, Vuadens O, Waldhauser K, Buetler TM, Kucera P, Ruegg UT (2006) Green tea extract and its major polyphenol (−)-epigallocatechin gallate improve muscle function in a mouse model for Duchenne muscular dystrophy. Am J Physiol Cell Physiol 290(2):C616–C625PubMedCrossRefGoogle Scholar
- 26.Chen KM, Lin JN, Lin HS, Wu HC, Chen WT, Li CH, Kai Lo S (2008) The effects of a Simplified Tai-Chi Exercise Program (STEP) on the physical health of older adults living in long-term care facilities: a single group design with multiple time points. Int J Nurs Stud 45(4):501–507PubMedCrossRefGoogle Scholar
- 46.Liang SY, Wu WC (1996) Tai Chi Chuan: 24 and 48 postures with martial applications. YMAA Publication Center, RoslindaleGoogle Scholar
- 47.Godin G, Shephard RJ (1997) Godin leisure time exercise questionnaire. Med Sci Sports Exerc 29:36–38Google Scholar
- 55.Tokuda H, Takai S, Matsushima-Nishiwaki R, Akamatsu S, Hanai Y, Hosoi T, Harada A, Ohta T, Kozawa O (2007) (−)-Epigallocatechin gallate enhances prostaglandin F2alpha-induced VEGF synthesis via upregulating SAPK/JNK activation in osteoblasts. J Cell Biochem 100(5):1146–1153PubMedCrossRefGoogle Scholar
- 58.Karabulut M, Bemben DA, Sherk VD, Anderson MA, Abe T, Bemben MG (2011) Effects of high intensity resistance training and low-intensity resistance training with vascular restriction on bone markers in older men. Eur J Appl Physiol [Epub ahead of print]Google Scholar
- 64.Flemiing KH, Heimbach JT (1994) Consumption of calcium in the U.S.: food sources and intake levels. J Nutr 124(8 Suppl):1426S–1430SGoogle Scholar
- 65.Fulgoni V 3rd, Nicholls J, Reed A, Buckley R, Kafer K, Huth P, DiRienzo D, Miller GD (2007) Dairy consumption and related nutrient intake in African-American adults and children in the United States: continuing survey of food intakes by individuals 1994–1996, 1998, and the National Health And Nutrition Examination Survey 1999–2000. J Am Diet Assoc 107(2):256–264PubMedCrossRefGoogle Scholar
- 67.Kemmler W, Lauber D, Weineck J, Hensen J, Kalender W, Engelke K (2004) Benefits of 2 years of intense exercise on bone density, physical fitness, and blood lipids in early postmenopausal osteopenic women: results of the Erlangen Fitness Osteoporosis Prevention Study (EFOPS). Arch Intern Med 164(10):1084–1091PubMedCrossRefGoogle Scholar
- 70.Canalis E, Hock JM, Raisz LG (1994) Anabolic and catabolic effects of parathyroid hormone on bone and interactions with growth factors. In: Bilezikian JP, Marcus R, Levine MA (eds) The parathyroids: basic and clinical concepts. Raven, New York, pp 65–82Google Scholar