Human bone material characterization: integrated imaging surface investigation of male fragility fractures
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The interrelation of calcium and phosphorus was evaluated as a function of bone material quality in femoral heads from male fragility fracture patients via surface analytical imaging as well as scanning microscopy techniques. A link between fragility fractures and increased calcium to phosphorus ratio was observed despite normal mineralization density distribution.
Bone fragility in men has been recently recognized as a public health issue, but little attention has been devoted to bone material quality and the possible efficacy in fracture risk prevention. Clinical routine fracture risk estimations do not consider the quality of the mineralized matrix and the critical role played by the different chemical components that are present. This study uses a combination of different imaging and analytical techniques to gain insights into both the spatial distribution and the relationship of phosphorus and calcium in bone.
X-ray photoelectron spectroscopy and time-of-flight secondary ion mass spectrometry imaging techniques were used to investigate the relationship between calcium and phosphorus in un-embedded human femoral head specimens from fragility fracture patients and non-fracture age-matched controls. The inclusion of the bone mineral density distribution via backscattered scanning electron microscopy provides information about the mineralization status between the groups.
A link between fragility fracture and increased calcium and decreased phosphorus in the femoral head was observed despite normal mineralization density distribution. Results exhibited significantly increased calcium to phosphorus ratio in the fragility fracture group, whereas the non-fracture control group ratio was in agreement with the literature value of 1.66 M ratio in mature bone.
Our results highlight the potential importance of the relationship between calcium and phosphorus, especially in areas of new bone formation, when estimating fracture risk of the femoral head. The determination of calcium and phosphorus fractions in bone mineral density measurements may hold the key to better fracture risk assessment as well as more targeted therapies.
KeywordsBackscattered scanning electron microscopy Bone mineral density distribution Calcium/phosphorus ratio Male fragility fractures Time-of-flight secondary ion mass spectrometry X-ray photoelectron spectroscopy
- 12.Eriksson C, Börner K, Nygren H, Ohlson K, Bexell U, Billerdahl N, Johansson aM (2006) Studies by imaging TOF-SIMS of bone mineralization on porous titanium implants after 1 week in bone. Proceedings of the fifteenth international conference on secondary ion mass spectrometry, 30 July 2006. -SIMS XV, Applied Surface Science, volume 252, issue 19, pp 6757–6760Google Scholar
- 16.Boivin G, Meunier PJ (2004) Inter-individual heterogeneity index of mineralization is an important determinant of the quality of bone. J Bone Miner Res 19:S114–S114Google Scholar
- 21.Zoehrer R, Roschger P, Paschalis EP, Hofstaetter JG, Durchschlag E, Fratzl P, Phipps R, Klaushofer K (2006) Effects of 3- and 5-year treatment with risedronate on bone mineralization density distribution in triple biopsies of the iliac crest in postmenopausal women. J Bone Miner Res 21:1106–1112CrossRefPubMedGoogle Scholar
- 22.Vickerman J, Briggs D, SurfaceSpectra (2001) ToF-SIMS: surface analysis by mass spectrometry. Chichester: IM, ManchesterGoogle Scholar
- 26.Vashishth D, Merle B, Gineyts E, Boivin G, Allen M, Burr DB, Delmas PD (2007) Effects of alendronate-induced bone matrix changes on resorption and turnover. J Bone Miner Res 22:S445–S445Google Scholar
- 27.Doublier A, Farla D, Khebbab MT, Jaurand X, Meunier PJ, Boivin G (2010) Bone mineral quality is maintained in osteoporotic women treated up to 60 months with strontium ranelate. Osteoporos Int 21:22–22Google Scholar
- 33.Bala Y, Chapurlat R, Delmas PD, Boivin G (2009) There is no hypermineralization among postmenopausal women receiving long-term oral bisphosphonates. Osteoporos Int 20:68–68Google Scholar
- 36.Fratzl-Zelman N, Roschger P, Gourrier A, Weber M, Misof BM, Loveridge N, Reeve J, Klaushofer K, Fratzl P (2009) Combination of nanoindentation and quantitative backscattered electron imaging revealed altered bone material properties associated with femoral neck fragility. Calcif Tissue Int 85:335–343CrossRefPubMedGoogle Scholar
- 37.Fratzl-Zelman N, Roschger P, Misof BM, Nawrot-Wawrzyniak K, Potter-Lang S, Muschitz C, Resch H, Klaushofer K, Zwettler E (2011) Fragility fractures in men with idiopathic osteoporosis are associated with undermineralization of the bone matrix without evidence of increased bone turnover. Calcif Tissue Int 88:378–387CrossRefPubMedGoogle Scholar