Osteoporosis International

, Volume 18, Issue 3, pp 385–390

Milk basic protein increases bone mineral density and improves bone metabolism in healthy young women

  • K. Uenishi
  • H. Ishida
  • Y. Toba
  • S. Aoe
  • A. Itabashi
  • Y. Takada
Original Article

DOI: 10.1007/s00198-006-0228-5

Cite this article as:
Uenishi, K., Ishida, H., Toba, Y. et al. Osteoporos Int (2007) 18: 385. doi:10.1007/s00198-006-0228-5


Effect of milk basic protein on bone metabolism in healthy young women.


Milk has more beneficial effects on bone health than other food sources. Recent in vitro and in vivo studies have shown that milk whey protein, especially its basic protein fraction (milk basic protein, MBP), contains several components capable of promoting bone formation and inhibiting bone resorption. The object of this study was to examine the effect of MBP on the bone mineral density and bone metabolism of healthy young women.


Thirty-five healthy young women were randomly assigned to treatment with either placebo or MBP (40 mg per day) for 6 months. The bone mineral density (BMD) of the lumbar vertebrae L2–L4 of each subject was measured by dual-energy X-ray absorptiometry (DXA) at 0 and 6 months of treatment. Serum and urine indexes of bone metabolism were measured at 0, 3 and 6 months. All subjects completed the study in accordance with the protocol.


The mean rate of gain of lumbar BMD in the MBP group (1.57%) was significantly higher than in the placebo group (0.13%, P=0.042). When compared with the placebo group, urinary cross-linked N-telopeptides of type-I collagen (NTx) were significantly decreased, and serum osteocalcin was significantly increased in the MBP group at 6 months.


These results suggested that MBP supplementation was effective in increasing BMD in young women and that this increase in BMD may be primarily mediated through the promotion of bone formation and inhibition of bone resorption by MBP supplementation.


Bone formation Bone mineral density Bone resorption Healthy young women Milk basic protein 

Copyright information

© International Osteoporosis Foundation and National Osteoporosis Foundation 2006

Authors and Affiliations

  • K. Uenishi
    • 1
  • H. Ishida
    • 2
  • Y. Toba
    • 3
  • S. Aoe
    • 4
  • A. Itabashi
    • 5
  • Y. Takada
    • 6
  1. 1.Laboratory of Physiological NutritionKagawa Nutrition UniversitySaitamaJapan
  2. 2.Laboratory of Administrative DieteticsKagawa Nutrition UniversitySaitamaJapan
  3. 3.Intellectual Property DepartmentSnow Brand Milk Products Co., Ltd.TokyoJapan
  4. 4.Department of Home EconomicsOtsuma Women’s UniversityTokyoJapan
  5. 5.Central Clinical LaboratorySaitama Medical SchoolSaitamaJapan
  6. 6.Product Planning DepartmentSnow Brand Milk Products Co., Ltd.SaitamaJapan

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