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No effect of topical application of tranexamic acid on articular cartilage

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Abstract

Purpose

The objective of this study was to evaluate potential cytotoxicity of TXA on articular cartilage by assessing chondrocyte viability of osteochondral explants after exposure to different concentrations and durations of TXA.

Methods

Thirty-nine osteochondral plugs (OCPs) were harvested from three adult Yucatan minipigs immediately after their death. OCPs were divided into 13 groups exposed to different concentrations of TXA (1, 2 and 4 mg/ml in saline solution) for 1, 3 and 6 h. Negative controls were exposed to saline solution for 0, 1, 3 and 6 h. Chondrocyte viability was assessed by Live/Dead cell assay and calculated as the ratio of live cells (green fluorescence) to overall cells (green + red cells) for each concentration of TXA and time point in a 50-µm scanned image.

Results

No correlation was found between chondrocyte viability, and TXA concentration and time of exposure. Overall, chondrocyte viability ranged from 90 to 99%. There was no statistical difference among control group, 1, 2 and 4 mg/ml TXA solutions at each time point [1 h (n.s.), 3 h (n.s.), 6 h (n.s.)]. Similarly, no statistical difference among groups was observed when comparing cell viability at 1, 3 and 6 h of TXA exposure, (Fig. 2) [1 mg/ml (n.s.), 2 mg/ml (n.s.), and 4 mg/ml (n.s.)].

Conclusions

In conclusion, doses of TXA approximating the current clinical protocols for topical use did not demonstrate any cytotoxic effects on cartilage explants in a Yucatan mini pig model. Thus, supporting the topical application for procedures with intact cartilage, such as partial knee replacement surgery and cartilage repair procedures.

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Fig. 2

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Author information

Correspondence to Luiz Felipe Ambra.

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Conflict of interest

All authors declare that they have no conflict of interest.

Funding

The authors received no financial support for the research, authorship, and/or publication of this article.

Ethical approval

This study was approved by the ethical committee of the Brigham Women’s Hospital.

Informed consent

This is an experimental study with no humans involved.

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Ambra, L.F., de Girolamo, L., Niu, W. et al. No effect of topical application of tranexamic acid on articular cartilage. Knee Surg Sports Traumatol Arthrosc 27, 931–935 (2019). https://doi.org/10.1007/s00167-017-4746-9

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Keywords

  • Cartilage
  • Tranexamic acid
  • Orthopedic surgery
  • Osteochondral
  • Animal study