All-arthroscopic AMIC procedure for repair of cartilage defects of the knee
- First Online:
- 1.4k Downloads
Bone marrow mesenchymal stem cells were introduced into clinical practice due to their ability to differentiate into many types of cells. Autologous matrix-induced chondrogenesis (AMIC) combines the microfracture method with matrix-based techniques that utilizes a collagen membrane to serve as a scaffold for new bone marrow mesenchymal stem cells, allowing effective reconstruction of even large fragments of a damaged cartilage surface.
All-arthroscopic technique to repair knee cartilage defects using the AMIC technique, which includes the use of a collagen matrix (porcine collagen type I and III) and fibrin glue—technique presentation.
This technical note introduces an all-arthroscopic AMIC technique to reconstruct extensive cartilage defects (without bone defects). The technique may be used for treatment of all location of knee cartilage lesions.
Level of evidence
KeywordsKnee Cartilage Collagen matrix All-arthroscopic
Various techniques, both palliative and reparative, have been used to treat cartilage pathology with variable success rates [1, 2, 3, 4, 5, 6, 7, 8]. One of the most promising fields of medical scientific research is regenerative medicine. Its major tools include both isolated cells and specially designed biomaterials. Bone marrow mesenchymal stem cells were introduced into clinical practice [3, 5, 7] due to their ability to differentiate into many types of cells [1, 5, 7]. In fact, bone marrow mesenchymal stem cells are considered to be an attractive source of cells for the regeneration of tissues and organs, including articular surface cartilage [1, 3, 5, 7]. The simplest method for utilizing stem cells in the regeneration of a damaged cartilage layer is the microfracture technique [1, 9]. Autologous matrix-induced chondrogenesis (AMIC) combines the microfracture method with matrix-based techniques that utilizes a collagen membrane to serve as a scaffold for new cells, allowing effective reconstruction of even large fragments of a damaged cartilage surface [2, 10, 11, 12, 13].
The goal of this article is to present an arthroscopic technique for reconstructing damaged knee cartilage fragments using the AMIC technique in conjunction with a collagen matrix (porcine collagen type I and III (Geistlich Pharma AG, Wolhusen, Switzerland)) and fibrin glue (Tissucol, Baxter, Warsaw, Poland).
This technical note describes first arthroscopic method to perform the AMIC procedure to treat cartilage defects in the knee. Technical progress has now made it possible to successfully apply arthroscopic techniques to reconstruct the damage of intra-articular knee structures (such as menisci or cruciate ligaments). The evolution of operative technique in knee cartilage repair from open to arthroscopic surgery is known also in autologous chondrocyte implantation . Marciacci et al. described all-arthroscopic technique for ACI-Hyalograft C, which is very similar with the technique presented in this paper . The surgical technique for ACI-Hyalograft C has 2 stages: cartilage biopsy and graft implantation, which is the most important difference with all-arthroscopic AMIC technique. Potential weakness of both all-arthroscopic ACI-Hyalograft C and AMIC technique versus open procedures is to use a few overlap circular patches of membrane fixed with glue. It is possible that the patches can move and expose bone. However, mobilization of the implanted patch has not been observed in Marcacci  and our series.
In the original AMIC technique (described by Benthien), fibrin glue was used to secure the membrane in the defect area [2, 10, 11]. However, others have reported the use of sutures to prevent membrane relocation. Considering the fact that good results were reported following the use of fibrin glue, the use of sutures appears to be superfluous, when only the cartilage surface needs to be reconstructed [2, 9, 10, 11]. Zantop et al. recommend to fix matrix with 1 or 2 biodegradable pins perpendicular to the surface . It is alternative to both gluing and suturing.
Stabilization of the Chondro-Gide membrane with glue requires a dry environment; so to complete the AMIC procedure, the fluid has to be removed before gluing the membrane. Some surgeons make use of special traction sets to generate these surgical spaces and placed patient in prone position . However, we found that with proper supine positioning, most cartilage defects on burdened knee joint surfaces are accessible for dry arthroscopy.
Here, we present all-arthroscopic technique to reconstruct extensive cartilage defects (without bone defects). The procedure is a single-stage, all- arthroscopic technique based on the open AMIC procedure. Clinical studies comparing this method with other methods or with active rehabilitation in randomized control trials is needed before the method can be recommended as a routine procedure.
This article is distributed under the terms of the Creative Commons Attribution Noncommercial License which permits any noncommercial use, distribution, and reproduction in any medium, provided the original author(s) and source are credited.
- 7.Sgaglione N, Miniaci A, Gillogly S, et al. (2002) Update on advanced surgical techniques in the treatment of traumatic focal articular cartilage lesions in the knee. Arthroscopy (Suppl 1)18:9–32Google Scholar
- 12.de Girolamo L, Bertolini G, Cervellin M, Sozzi G, Volpi P (2010) Treatment of chondral defects of the knee with one step matrix-assisted technique enhanced by autologous concentrated bone marrow: in vitro characterisation of mesenchymal stem cells from iliac crest and subchondral bone. Injury 41:1172–1177PubMedCrossRefGoogle Scholar
- 13.Dhollander AA, De Neve F, Almqvist KF, Verdonk R, Lambrecht S, Elewaut D, Verbruggen G, Verdonk PC (2011) Autologous matrix-induced chondrogenesis combined with platelet-rich plasma gel: technical description and a five pilot patients report. Knee Surg Sports Traumatol Arthrosc 19:536–542PubMedCrossRefGoogle Scholar
- 14.Siebold R, Sartory N, Yang Y, Feil S, Paessler HH (2011) Prone position for minimal invasive or all-arthroscopic autologus chondrocyte implantation at the patella. Knee Sur, Sports Traumatol Arthrosc Published online: 09 AprilGoogle Scholar