Knee Surgery, Sports Traumatology, Arthroscopy

, Volume 18, Issue 12, pp 1679–1684 | Cite as

The effect of microRNA-21 on proliferation and matrix synthesis of chondrocytes embedded in atelocollagen gel

  • Wirat Kongcharoensombat
  • Tomoyuki Nakasa
  • Masakazu Ishikawa
  • Atsuo Nakamae
  • Masataka Deie
  • Nobuo Adachi
  • Abouheif Mohamed
  • Mitsuo Ochi
Experimental Study
First Online:
Received:
Accepted:

Abstract

The objective of this study was to investigate the effect of microRNA-21 (miR-21) on the proliferation and matrix synthesis of chondrocytes embedded in atelocollagen gel. Articular cartilage was harvested aseptically from the knee and hip joints of rats. In the experimental group, double-stranded miR-21 was transfected into the chondrocytes, and in the control group, scrambled siRNA was used. After that, chondrocytes were cultured in atelocollagen gel for 3 weeks. At 1, 2, and 3 weeks after transfection, the cell numbers were counted, and the expression levels of Col2a1 and aggrecan were measured by real-time PCR. Histological analysis by toluidine blue staining was performed at 3 weeks. The cell number in the experimental group rapidly increased compared to the control group. The expression levels of Col2a1 and aggrecan in the experimental group were higher than in the control. Histological analysis revealed that many more cells with a metachromatic stain were present in the experimental group than in the control. This study demonstrated that miR-21 promotes high proliferation and matrix synthesis of chondrocytes embedded in atelocollagen gel.

Keywords

MicroRNA-21 Chondrocytes Atelocollagen gel Three-dimensional culture Gene expression 
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Notes

Conflict of interest statement

None of the authors have received any financial support from any organizations to influence this study.

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Copyright information

© Springer-Verlag 2010

Authors and Affiliations

  • Wirat Kongcharoensombat
    • 1
  • Tomoyuki Nakasa
    • 1
  • Masakazu Ishikawa
    • 1
  • Atsuo Nakamae
    • 1
  • Masataka Deie
    • 1
  • Nobuo Adachi
    • 1
  • Abouheif Mohamed
    • 1
  • Mitsuo Ochi
    • 1
  1. 1.Department of Orthopaedic Surgery, Programs for Applied Biomedicine, Division of Clinical Medical Science, Graduate School of Biomedical SciencesHiroshima UniversityMinami-kuJapan

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