Effect of dihydrotestosterone on cultured human tenocytes from intact supraspinatus tendon
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The role of hormones in the pathogenesis of tendinopathy is not well recognised, even though the use of anabolic steroids is correlated with a higher incidence of spontaneous tendon ruptures. The aim of this study was to investigate the effects of dihydrotestosterone (DHT) on human tenocyte cultures from the intact supraspinatus tendon of male subjects. Cultured human tenocytes were seeded into culture plates at a density of 5 × 104 cells per well and incubated for 24 h. Then, 10−9 M–10−7 M DHT or Dulbecco’s modified Eagle’s medium (DMEM) only (control) was added to the culture plate wells. Cell morphology assessment and cell proliferation tests were performed 48, 72 and 96 h after DHT treatment. DHT-treated tenocytes showed an increased proliferation rate at DHT concentration higher than 10−8 M. Differences in cell numbers between control and DHT-treated cells were statistically significant (P < 0.05) after 48 and 72 h of treatment with DHT concentrations of 10−8 and 10−7 M. The tenocytes treated with DHT (10−8 and 10−7 M) became more flattened and polygonal compared to control cells that maintained their fibroblast-like appearance during the experiment at each observation time. In conclusion, in vitro, progressive increasing concentration of DHT at doses greater than 10−8 M had direct effects on male human tenocytes, increasing cell number after 48 and 72 h of treatment, and leading to a dedifferentiated phenotype after 48 h of treatment. This effect can be important during tendon-healing and repair, when active proliferation is required. Our results represent preliminary evidence for a possible correlation between testosterone abuse and shoulder tendinopathy.
KeywordsTenocytes Dihydrotestosterone Anabolic steroids Tendinopathy Shoulder Sports
Conflict of interest statement
The authors declare no conflict of interest.
- 5.Benz DJ, Haussler MR, Thomas MA, Speelman B, Komm BS (1991) High-affinity androgen binding and androgenic regulation of alpha 1(I)-procollagen and transforming growth factor-beta steady state messenger ribonucleic acid levels in human osteoblast-like osteosarcoma cells. Endocrinology 128:2723–2730CrossRefPubMedGoogle Scholar
- 14.Franceschi F, Longo UG, Ruzzini L, Rizzello G, Maffulli N, Denaro V (2008) No advantages in repairing a type II superior labrum anterior and posterior (SLAP) lesion when associated with rotator cuff repair in patients over age 50: a randomized controlled trial. Am J Sports Med 36:247–253CrossRefPubMedGoogle Scholar
- 26.Knobloch K, Schreibmueller L, Longo UG, Vogt PM (2008) Eccentric exercises for the management of tendinopathy of the main body of the Achilles tendon with or without an AirHeeltrade mark Brace. A randomized controlled trial. B: effects of compliance. Disabil Rehabil 30:1692–1696CrossRefPubMedGoogle Scholar
- 27.Knobloch K, Schreibmueller L, Longo UG, Vogt PM (2008) Eccentric exercises for the management of tendinopathy of the main body of the Achilles tendon with or without the AirHeeltrade mark Brace. A randomized controlled trial. A: effects on pain and microcirculation. Disabil Rehabil 30:1685–1691CrossRefPubMedGoogle Scholar